Progesterone decrease plasma membrane in human sperm with subnormal hypoosmotic swelling test scores
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Abstract
Progesterone (P4) is known as a female hormone affecting oocyte maturation and developing uterine wall. A proteomic study identified several receptors including P4 receptors on human sperm. The role of P4 in human sperm cells remains unknown as to whether P4 has non-genomic effects on human sperm. The present study aims to determine the effect of progesterone (P4) on the hyperactivated motility and membrane integrity of human sperm cells.
Methods
Semen from normal individuals was obtained from donors. The semen was washed by gradient density centrifugation. P4 was added to each semen sample to final concentrations of 0 (control), 250, 500, 750 and 1000 ng/mL. After the sample treatment was completed, the sperm membrane integrity was assessed with the hypoosmotic swelling test (sodium citrate dihydrate and D-fructose) and the hyperactivated sperm motility parameter was determined with the Computer Assisted Sperm Analyzer [CASA] (Hamilton Thorne, IVOS II, USA). The percentage was then compared between the treatment groups and the control group. The percentage differences were analyzed with the Sigmastat version 2.0 statistical program.
Results
Administration of P4 increased sperm hyperactivated motility when compared with the control group at a concentration of 500 ng/mL, but the increase was statistically not signicant (p>0.05). In contrast, P4 decreased sperm membrane integrity significantly (p=0.042). And the mean of plasma membrane integrity in all groups was subnormal hypoosmotic swelling test score.
Conclusion
Progesterone administration tends to increase sperm hyperactivated motility. The integrity of plasma sperm membrane was affected by progesterone.
Article Details
The journal allows the authors to hold the copyright without restrictions and allow the authors to retain publishing rights without restrictions.
References
Lishko PV, Botchkina IL, Kirichok Y. Progesterone activates the principal Ca2+ channel of human sperm. Nature 2011;471:387-91. doi: 10.1038/nature09767.
Meharvan S, Chang S, Selena N. Non-genomic mechanisms of progesterone action in the brain. Front Neurosci 2013;7:159. doi: 10.3389/fnins.2013.00159.
Alasmari W, Costello S, Correia J, et al. Ca2+ signals generated by CatSper and Ca2+ stores regulate different behaviour in human sperm. J Biol Chem 2013;288:6248-58. doi: 10.1074/jbc.M112.439356.
Strünker T, Goodwin N, Brenker C, et al. The CatSper channel mediates progesterone induced Ca2+ influx in human sperm. Nature 2011;471:382-6. doi: 10.1038/nature09769.11.
Baker MA, Hetherington L, Reeves GM, et al. The mouse sperm proteome characterized via IPG strip prefractionation and LC-MS/MS identification. Proteomic 2008;8:1720-30. doi: 10.1002/pmic.200701020.
Sayme N, Dite L, Krebs T, et al. Positive effect of progesterone on motility and velocity of fresh, vitrified without permeable cryoprotectants and frozen with permeable cryoprotectans human spermatozoa. Andrologia 2018;e13133:1-7. doi: 10.1111/and.13133
Wiser A, Sachar S, Ghetler Y, et al. Assessment of sperm hyperactivated motility and acrosome reaction can discriminate the use of spermatozoa for conventional in vitro fertilisation or intracytoplasmic sperm injection: preliminary results. Andrologia 2013;46:313-5. doi: 10.1111/and.12068.
Ramu S, Jeyendran RS. The hypo-osmotic swelling test for evaluation of sperm membrane integrity. In: Carrell D, Aston K, editors. Spermatogenesis. methods in molecular biology (methods and protocols). Totowa NJ: Humana Press;2013.p.21-5.
World Health Organization, Department of Reproductive Health and Research. WHO laboratory manual for the examination and processing of human semen. 5th ed. Geneva: World Health Organization, Department of Reproductive Health and Research;2010.
Perdrix A, Saidi R, Menard JF, et al. Relationship between conventional sperm parameters and motile sperm organelle morphology examination (MSOME). Int J Androl 2012;35:491-8. doi: 10.1111/j.1365-2605.2012.01249.x.
Cooper TG, Noonan E, von Eckardstein S, et al. World Health Organization reference values for human semen characteristics. Hum Reprod Update 2010;16:231-45. doi: 10.1093/humupd/dmp048.
Lishko PV, Botchkina IL, Kirichok Y. Progesterone activates the principal Ca2+ channel of human sperm. Nature 2011;471:387–91. doi:10.1038/nature0976710.
Sumigama S, Mansell S, Miller M, et al. Progesterone accelerates the completion of sperm capacitation and activates CatSper channel in spermatozoa from the Rhesus macaque. Biol Reprod 2015;93:1-11. doi: 10.1095/biolreprod.115.129783.
Rahman MS, Kwon WS, Pang MG. Calcium influx and male fertility in the context of the sperm proteome: an update. Biomed Res Int 2014;1-13. doi: 10.1155/2014/841615.
Agarwal A, Virk G, Ong C, et al. Effect of oxidative stress on male reproduction. World J Mens Health 2014;32:1-17. doi: 10.5534/wjmh.2014.32.1.1.
Breznik BP, Kovacic B, Vlaisavljevic V. Are sperm DNA fragmentation, hyperactivation and hyaluronan-binding ability predictive for fertilization and embryo development in in vitro fertilization and intracytoplasmic sperm injection? Fertil Steril 2013;99:1233-41. doi: 10.10.16/j.fertnstert.2013.11.048.
Lodhi LA, Zubair M, Qureshi ZI, et al. Correlation between hypo-osmotic swelling test and various conventional semen evaluation parameters in fresh Nili Ravi buffalo and Sahiwal cow bull semen. Pak Vet J 2008;28:186–8.
Duru NK, Morshedi M, Schuffner A, et al. Semen treatment with progesterone and/or acetyl-L-carnitine does not improve sperm motility or membrane damage after cryopreservation-thawing. Fertil Steril 2000;74:715-20.
Smith JF, Syritsyna O, Fellous M, et al. Disruption of the pricipal, progesterone-activated sperm Ca2+ channel in a CatSper2-deficient infertile patient. Proc Natl Acad Sci USA 2013;110:6823-8.
Henning H, Ngo TT, Waberski D. Centrifugation stress reduces the responsiveness of spermatozoa to a capacitation stimulus in in vitro-aged semen. Andrology 2015;3:834-42. doi: 10.1111/andr.12064.
Mohammadi G, Mahdion H. Evaluation of membrane integrity of bull frozen-thawed sperm using water and hypo osmotic swelling test. Bas J Vet Res 2017;16:131-43.
Youn JS, Cha SH, Park CW, et al. Predictive value of sperm motility characteristics assessed by computer assisted sperm analysis in intrauterine insemination with superovulation in couples with unexplained infertility. Clin Exp Reprod Med 2011. 38:47-52. doi: 10.5653/cerm.2011.38.1.47.