@article{Kuswati_Prakosa_Wasita_Wiyono_2015, title={Centella asiatica increases B-cell lymphoma 2 expression in rat prefrontal cortex}, volume={34}, url={https://univmed.org/ejurnal/index.php/medicina/article/view/17}, DOI={10.18051/UnivMed.2015.v34.10-16}, abstractNote={<p>BACKGROUND<br />Stress is one of the factors that cause apoptosis in neuronal cells. Centella<br />asiatica has a neuroprotective effect that can inhibit apoptosis. This study<br />aimed to examine the effect of Centella asiatica ethanol extract on B-cell<br />lymphoma 2 (Bcl-2) protein expression in the prefrontal cortex of rats.</p><p>METHODS<br />An experimental study was conducted on 34 brain tissue samples from male<br />Sprague Dawley rats exposed to chronic restraint stress for 21 days. The<br />samples were taken from following groups: non-stress group K, negative<br />control group P1 (stress + arabic gum powder), P2 (stress + C.asiatica at<br />150 mg/kgBW), P3 (stress + C.asiatica at 300 mg/kg BW), P4 (stress +<br />C.asiatica at 600 mg/kg body weight) and positive control group P5 (stress<br />+ fluoxetine at 10 mg/kgBW). The samples were made into sections that<br />were stained immunohistochemically using Bcl-2 antibody to determine the<br />percentage of cells expressing Bcl-2. Data were analyzed using one way<br />ANOVA test followed by a post - hoc test.</p><p>RESULTS<br />There were significant differences in mean Bcl-2 expression between the<br />groups receiving Centella asiatica compared with the non-stress group and<br />stress-only group (negative control group) (p<0.05). The results were<br />comparable to those of the fluoxetine treatment group.</p><p>CONCLUSION</p><p>The Centella asiatica ethanol extract was able to increase Bcl-2 expression<br />in the prefrontal cortex of Sprague Dawley rats exposed to restraint stress.<br />This study suggests that Centella asiatica may be useful in the treatment of<br />cerebral stress.</p>}, number={1}, journal={Universa Medicina}, author={Kuswati, Kuswati and Prakosa, Djoko and Wasita, Brian and Wiyono, Nanang}, year={2015}, month={Apr.}, pages={10–16} }