TY - JOUR AU - Sumarsono, Portia AU - Widjiati, Widjiati AU - Susilowati, Suherni PY - 2016/08/16 Y2 - 2024/03/29 TI - Red fruit oil increases trophoblast cells and decreases caspase-9 expression in placenta of lead exposed mice JF - Universa Medicina JA - Univ Med VL - 35 IS - 2 SE - Original Articles DO - 10.18051/UnivMed.2016.v35.110-118 UR - https://univmed.org/ejurnal/index.php/medicina/article/view/165 SP - 110-118 AB - BACKGROUND<br />Lead is able to pass through the placental barrier and interfere with fetal development. Red fruit has high antioxidant activity, due to carotenoids (pro-vitamin A), tocopherols (vitamin E) and unsaturated fatty acids. This study aims to examine the effect of red fruit oil towards placenta in pregnant mice before lead exposure.<br /><br />METHODS<br />This was a laboratory experimental post test only study, using 20 pregnant mice (Mus musculus) that were randomized into five groups. The negative control group was not exposed to lead; intervention group 0 (P0) was exposed to lead at 0.011 mg/20 g BW/day on days 6-15 of gestation. The intervention groups P1-P3 were given red fruit orally at 0.3 mL/20 g BW/day, 0.8 mL/20 g BW/day, 0.9 mL/20 g BW/day, respectively, before lead exposure. Normal trophoblast cell count and caspase-9 expression of trophoblasts were calculated. One-way ANOVA, Kruskal-Wallis and Mann Whitney tests were used to analyze the data.<br /><br />RESULTS<br />The intervention groups P2 and P3 given red fruit at 0.8 mL/20 g BW/day and 0.9 mL/20 g BW/day showed a significant increase in normal trophoblast cell count of 88.2% (p=0.024) and a decrease in caspase-9 expression of 70.8% compared with P0 to which was not given red fruit (p=0.020). <br /><br />CONCLUSION<br />Red fruit increased normal trophoblast cell count by 88.2% and decreased caspase-9 expression by 70.8% in pregnant mice before lead exposure. It should be noted that red fuit may prevent oxidative damage to cells. ER -