Zinc supplementation improves heme biosynthesis in rats exposed to lead

Main Article Content

Budi Santoso
Hertanto Wahyu Subagio
Lisyani Suromo
Henna Rya Sunomo


Lead acetate (Pb) inhibits heme biosynthesis through inhibition of δ-aminolevulinic acid dehydrogenase (δ-ALAD), copro porphyrinogen
oxidase, and ferro chelatase. Zinc supplementation increases lead-binding
metallothionein proteins. The purpose of this study was to find evidence that zinc supplementation prior to lead exposure improves heme biosynthesis in rats

This was a randomized post-test only control-group design study involving 28 rats assigned to 4 groups (1 control and 3 treatment groups). The treatment groups were supplemented with zinc at doses of 0.2, 0.4, and
0.8 mg daily by gavage for 3 weeks. From week 4 to 13, all groups were
exposed to lead 0.5 g/kg BW/day by gavage. At the end of week 13, δ-
ALAD, erythrocyte protoporphyrin (EPP), and heme concentrations were
determined by means of ELISA. One-way ANOVA, followed by
Bonferroni’s test was used to analyse the data.

Mean δ-ALAD concentrations decreased from the control group down to
treatment group 3 (0.24 ± 0.20; 0.15 ± 0.15; 0.12 ± 0.11; 0.05 ± 0.06 ng/
mean per unit). Mean EPP concentrations decreased from the control group down to treatment group 3 (1.96 ± 0.50; 1.24 ± 0.24; 1.03 ± 0.05; 0.62 ± 0.16 ng/mL). Mean heme concentrations increased from the control
group up to treatment group 3 (8.07 ± 2.64; 10.11 ± 2.27; 10.04 ± 1.65;
11.41 ± 2.58 μM). ANOVA followed by Bonferroni showed that EPP concentrations differed significantly between the control group and treatment group 3 (p=0.00).

Zinc supplementation prior to lead exposure improves heme biosynthesis
in rats exposed to lead.

Article Details

How to Cite
Santoso, B., Subagio, H. W., Suromo, L., & Sunomo, H. R. (2015). Zinc supplementation improves heme biosynthesis in rats exposed to lead. Universa Medicina, 34(1), 3–9. https://doi.org/10.18051/UnivMed.2015.v34.3-9
Review Article
Author Biographies

Budi Santoso, Health Analyst, Faculty of Nursing and Health, Muhammadiyah University, Semarang

Health Analyst,

Hertanto Wahyu Subagio, Department of Nutrition, Dr. Kariadi Hospital/ Diponegoro University, Semarang

Department of Nutrition,

Lisyani Suromo, Department of Pathology, Dr. Kariadi Hospital/ Diponegoro University, Semarang

Department of Pathology,

Henna Rya Sunomo, Department of Pharmacy, Dr. Kariadi Hospital/ Diponegoro University, Semarang

Department of Pharmacy


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