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Real time polymerase chain reaction for detecting SARS-COV-2 in Indonesia: are the results reliable?

Monica Dwi Hartanti
Submission date: Thursday, 07 May 2020
Published date: Wednesday, 17 June 2020
DOI: http://dx.doi.org/10.18051/UnivMed.2020.v39.71-73

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Abstract


Since it was first reported in Wuhan, China in December 2019, coronavirus disease 2019 (COVID-19) has been considered as a pandemic. It is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a member of the Coronavirus family. In Indonesia, this disease first detected in the middle of March 2020 and it was spreading all over Indonesia. The Indonesian Government has implemented several strategies in order to detain the spreading of COVID-19, including improving diagnostic management. Until now, the most reliable method of detecting COVID-19 is real time-PCR.


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References


World Health Organization. COVID 19 public health emergency of international concern (PHEIC). Geneva: World Health Organization;2020.

Corona Disease Mitigation Acceleration Task Force. COVID-19 in Indonesia (2 June 2020). Jakarta: Corona Disease Mitigation Acceleration Task Force;2020.

Hasell J, Ortiz-Ospina E, Mathieu E, et al. Coronavirus (COVID-19) testing. Our World in Data;2020.

Saiki RK, Scharf S, Faloona F, et al. Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science 1985;230:1350-4. doi: 10.1126/science.2999980.

Shen M, Zhou Y, Ye J, et al. Recent advances and perspectives of nucleic acid detection for coronavirus. J Pharm Anal 2020;10:97-101. https://doi.org/10.1016/j.jpha.2020.02.010.

World Health Organization. Laboratory testing for coronavirus disease 2019 (COVID-19) in suspected human cases: interim guidance. Geneva: World Health Organization;2020.

Wang Y, Kang H, Liu X, Tong Z. Combination of RT-qPCR testing and clinical features for diagnosis of COVID-19 facilitates management of SARS-CoV-2 outbreak. J Med Virol 2020;1 -2.doi: 10.1002/jmv.25721.

Xiao AT, Tong YX, Gao C, et al. Dynamic profile of RT-PCR findings from 301 COVID-19 patients in Wuhan, China: a descriptive study. J Clin Virol 2020;104346.https://doi.org/10.1016/j.jcv.2020.104346.

Forster P, Forster L, Renfrew C, Forster M. Phylogenetic network analysis of SARS-CoV-2 genomes. Proc Natl Acad Sci USA 2020; 117:9241-3. doi: 10.1073/pnas.2004999117.

Global Initiative on Sharing All Influenza Data. Genomic epidemiology of hCoV-19. Bonn: Global Initiative on Sharing All Influenza Data (GISAID), 2020.

Pengurus Pusat Perhimpunan Dokter Spesialis Patologi Klinik dan Kedokteran Laboratorium Indonesia. Manajemen spesimen dan diagnosis laboratorium kasus suspek 2019-nCoV;2020.


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Copyright (c) 2020 Monica Dwi Hartanti

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This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

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Universa Medicina by Faculty of Medicine, Trisakti University is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Based on a work at https://univmed.org/ejurnal/index.php/medicina/


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