Bone microstructure and atomic periodic disharmonization in osteoporosis

Main Article Content

Zairin Noor
Mohammad Hidayat
Agus Hadian Rahim
Sutiman B. Sumitro

Abstract

Background
Both cortical and cancellous bone display a complex, porous microstructure whose properties depend on the macrostructure of bone as well as age and health of the individual. The aim of this study was to compare the microstructure and characteristics of mineral atoms in osteoporotic and normal bone.

Methods
A prospective laboratory experimental study was conducted from August to December 2010 at several hospitals in Banjarmasin. Twenty patients with osteoporosis and twenty six normal patients were involved in this study. Bone obtained from surgery was analyzed for microstructure by scanning electron microscopy (SEM), while mapping of mineral atoms was performed by means of SEM-Energy dispersive X-ray spectroscopy (SEM-EDAX) at the Brawijaya University, Malang.
    
Results
The osteoporotic subjects with mean age of 64.65 ± 16.41 years were older than the normal bone subjects with mean age of 39.38 ± 17.16 years. The body mass index was similar in both groups of subjects. From SEM-EDAX results, three patterns of mineral atoms were apparent in osteoporotic and normal bone. In osteoporotic bone, these patterns were indicative of a disordered substitution or incorporation process. SEM results showed degeneration of microarchitecture (resorption cavities, perforations, and prominent granules) in osteoporotic but not in normal bone. There was a significant difference in microstructure between osteoporotic and normal bone, which was caused by differences in atomic properties.
    
Conclusions
Microstructural abnormalities of bone and disharmonization of mineral atoms in the periodic system were found in osteoporosis.

Article Details

How to Cite
Noor, Z., Hidayat, M., Rahim, A. H., & Sumitro, S. B. (2012). Bone microstructure and atomic periodic disharmonization in osteoporosis. Universa Medicina, 31(2), 96–104. https://doi.org/10.18051/UnivMed.2012.v31.96-104
Section
Review Article

References

Mano JF. Viscoelastic properties of bone: mechanical spectroscopy studies on a chicken model. Mater Sci Eng 2005;25:145-52.

Tho JY, Zioupos P, Currey JD, Pharr GM. Microstructural elasticity and regional heterogeneity in human femoral bone of various ages examined by nano-indentation. J Biomech 2002;35:189-98.

Huang Q, Kung AWC. Genetic of osteoporosis. Mol Genet Metab 2006;88:295-306.

Duncan EL, Brown MA. Genetic studies in osteoporosis – the end of the beginning. Arthritis Res Ther 2008;10:214.

Brandao CMR, Lima MG, da Silva AL, Silva GD, Guerra AA Jr, Acurcio FA. Treatment of postmenopausal osteoporosis in women: a systematic review. Cad Saude Publica 2008; 24Supl4:592-606.

Handa R, Kalla AA, Maalouf G. Osteoporosis in developing countries. Best Pract Res Clin Rheumatol 2008;22:693-708.

Wiechula D, Jurkiewicz A, Loska K. An assessment of natural concentrations of selected metals in the bone tissue of the femur head. Sci Total Environ 2008;406:261-7.

Nielsen FH, Stoecker BJ. Boron and fish oil have different beneficial effects on strength and trabecular microarchitecture of bone. J Trace Elem Med Biol 2009;23:195-203.

Odabasi E, Turan M, Aydin A, Akay C, Kutlu M. Magnesium, zinc, copper, manganese, and selenium levels in postmenopausal women with osteoporosis: can magnesium play key role in osteoporosis. Ann Acad Med Singapore 2008; 37:564-7.

Noor Z, Sumitro SB, Hidayat M, Rahim AH, Taufiq A. Assesment of microarchitecture and crystal structure of hydroxyapatite in osteoporosis. Univ Med 2011;30:29-35.

Sastry TP, Chandrasekaran A, Sundaraseelan J, Ramasastry M, Sreedhar R. Comparative study of some physico-chemical characteristics of osteoporosis and normal human femur heads. Clin Biochem 2007;40:907-912.

Shen Y, Zhang Z, Jiang S, Jiang L, Dai L. Postmenopausal woman with osteoarthritis and osteoporosis show different ultrastructural characteristics of trabecular bone of the femoral head. BMC Musculoskeletal Disord 2009;10:35. doi:10.1186/1471-2474-10-35.

Jasiuk IM. Analysis of trabecular bone as a hierarchial material. XXI ICTAM, Warsaw, Poland, 15-21 August, 2004.

Noor Z, Sumitro SB, Hidayat M, Rahim AH, Sabarudin A, Umemura T. Atomic mineral characteristics of Indonesian osteoporosis by high-resolution inductively coupled plasma mass spectrometry. The Sci World J 2012. doi: 10.1100/2012/372972.

Vallet-Regi M, Arcos D. Biomimetic nanoceramics in clinical use: from materials to applications. Cambridge: The Royal Society of Chemistry, Thomas Graham House, Science Park;2008.

Ovesen J, Moller-Madsen B, Nielsen PT, Christensen PH, Simonsen O, Hoeck HC, et al. Differences in zinc status between patients with osteoarthritis and osteoporosis. J Trace Elem Med Biol 2009;23:1-8.

Holloway WR, Collier FM, Herbst RE. Hodge JM, Nicholson GC. Osteoblast-mediated effects of zinc on isolated rat osteoclasts: inhibition of bone resorption and enhancement of osteoclast number. Bone 1996;19:137–42.

Aina V, Perardi A, Bergandi L. Cytotoxicity of zinc-containing bioactive glasses in contact with human osteoblasts. Chem Biol Interact 2007; 167:207-18.

Busse D, Hahn M, Soltau M, Zustin J, Puschel K, Duda GN, et al. Increased calcium content and inhomogeneity of mineralization render bone toughness in osteoporosis: mineralization, morphology and biomechanics of human single trabeculae. Bone 2009;45:10341-3.