Nephroprotective effect of soy (Glycine Max (L.) Merr.) flour against diazinon-induced renal toxicity in rats

Main Article Content

Desie Dwi Wisudanti
Rena Normasari
Firman Herdiyana

Abstract

Background
Diazinon is a widely used pesticide to eradicate agricultural pests globally with the potential to cause harmful effects on humans by generating free radicals and depleting endogenous antioxidants. Soy flour possesses high antioxidant effect. This study evaluated the nephroprotective effect of soy flour  on diazinon-induced nephrotoxicity in rats.

Methods
An experimental laboratory study was carried out involving 30 male Wistar rats were randomly divided into five groups of 6 rats. The normal control (Kn) and the negative control (K-) groups received normal saline, the treatment groups (K1, K2, K3) received 10%, 15%, and 20% soy flour in distilled water, respectively, on day 1 to day 28. Subsequently, for five days, all groups received diazinon 40 mg/kg, except for the normal control group. Blood urea nitrogen (BUN) and creatinine levels were measured at the end of the study. The Kruskal Wallis test with the post hoc Mann Whitney U test were used to analyze the data.

Results
Blood urea nitrogen and creatinine levels were lower in all treatment groups (K1, K2, K3) compared to the negative control group (K-), while the K3 level was the lowest (24.28 ± 1.56 mg/dL and 0.93 ± 0.14 mg/dL).  There was a significant decrease in BUN and creatinine levels in the experimental rats of the 15% and 20% soy flour groups (K2, K3) compared to the negative control group (K-).

Conclusion
Administration of soy flour (Glycine max (L.) Merr.) had a nephroprotective effect in preventing increases in BUN and creatinine levels of diazinon-induced nephrotoxic male Wistar rats.

Article Details

How to Cite
Wisudanti, D. D., Normasari, R., & Herdiyana, F. (2020). Nephroprotective effect of soy (Glycine Max (L.) Merr.) flour against diazinon-induced renal toxicity in rats. Universa Medicina, 39(3), 192–198. https://doi.org/10.18051/UnivMed.2020.v39.192-198
Section
Original Articles
Author Biographies

Desie Dwi Wisudanti, Department of Pharmacology, Faculty of Medicine, University of Jember

Department of Pharmacology

Rena Normasari, Department of Pathology Anatomy, Faculty of Medicine, University of Jember

Department of Pathology Anatomy

Firman Herdiyana, Undergraduate Program, Faculty of Medicine, University of Jember

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References

Suryana A. Menuju ketahanan pangan Indonesia berkelanjutan 2025: tantangan dan penanganannya. Forum Penelit Agro Ekon 2014;32:123–35. doi: 10.21082/fae.v32n2.2014.123-125.

Terry AV. Functional consequences of repeated organophosphate exposure: potential non-cholinergic mechanisms. Pharmacol Ther 2012;134:355–65. doi: 10.1016/j.pharmthera.2012. 03.001.

Azis T. Analisis residu pestisida diazinon dalam tanaman kubis (Brassica olarecea) menggunakan biosensor elektrokimia secara voltametri siklik. J Prog Kim Sains 2011;1: 32–40.

Sarhan OMM. Histological and biochemical effects of diazinon on liver and kidney of rabbits. Life Sci J 2011;8: 1183–9.

Wisudanti DD, Herdiana F, Qodar TS. Diazinon toxicity to kidney and liver of Wistar male rats in terms of biochemical and histopathological parameters. J Agromedicine Med Sci 2019;5: 112-7. doi: https://doi.org/10.19184/ams.v5i2.11575.

Sargazi Z, Nikravesh MR, Jalali M, Sadeghnia HR. Apoptotic effect of organophosphorus insecticide diazinon on rat ovary. Iran J Toxicol 2016;10: 37–44.

Cakici O, Akat E. Effects of oral exposure to diazinon on mice liver and kidney tissues: biometric analyses of histopathologic changes. Anal Quant Cytol Histol 2013;35:7–16.

Kumar S, Pandey AK, Lu KP, Sastre J. Chemistry and biological activities of flavonoids: an overview. Sci World J 2013;2013:16. doi: 10.1155/2013/162750.

Sharma P, Jha AB, Dubey RS, Pessarakli M. Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions. J Bot 2012;2012:1-26. doi: 10.1155/2012/217037.

Liang Y, Qi W, Guo W, Wang C, Hu Z, Li A. Genistein and daidzein induce apoptosis of colon cancer cells by inhibiting the accumulation of lipid droplets. Food Nutr Res 2018;62:1384-93. doi: 10.29219/fnr.v62.1384.

Okafor HH, Ebuehi OAT. Defatted soya flour supplementation of wheat bread confers oxidative, renal, hepatic and cardiovascular protective effects in Wistar rats. Int J Biochem Res Rev 2016;10:1–4. doi: 10.9734/IJBCRR/2016/22912.

Khan TH. Soy diet diminish oxidative injure and early promotional events induced by CC14 in rat liver. Int J Pharmacol 2012;8:30–8. doi: 10.3923/ijp.2012.30.38.

Charan J, Kantharia N. How to calculate sample size in animal studies? J Pharmacol Pharmacother 2013;4:303–6. doi: 10.4103/0976-500X.119726.

Shah MD, Iqbal M. Diazinon-induced oxidative stress and renal dysfunction in rats. Food Chem Toxicol 2010;48:3345–53. doi: 10.1016/j.fct.2010. 09.003.

Al-Ghanim KA. Effect of an organophosphate insecticide diazinon on the activity of acetylcholinesterase and lipid peroxidation of a common carp, Cyprinus carpio L. Pakistan J Zool 2014;46:161-6.

Ayala A, Munoz MF, Arguelles S. Lipid peroxidation: production, metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal. Oxid Med Cell Longev 2014;2:1-31. doi: 10.1155/2014/360438.

Rahimi F, Nikravesh MR, Jalali M, Sadeghnia HR, Sargazi Z. The effect of diazinon on cell proliferation and apoptosis in testicular tissue of rats and the protective effect of vitamin E. Int J Fertil Steril 2019;13:154-60. doi:10.22074/ijfs.2019.5612.

Sanz A, Santamaria B, Ruiz-Ortega M, Egido J, Ortiz A. Mechanisms of renal apoptosis in health and disease. J Am Soc Nephrol 2008;19:1634-42.

Qodar TS, Wisudanti DD, Aziz AM. Efek pemberian tepung kedelai terhadap gambaran histopatologi ginjal tikus Wistar yang diinduksi diazinon. eJournal Kedokt Indones 2019;7:66-71. doi: 10.23886/ejki.7.10287.

Bhagwat S, Haytowitz D B, Holden JM. USDA database for the isoflavone content of selected foods. U.S. Department of Agriculture, Nutrient Data Laboratory; 2011.

Niki E. Evidence for beneficial effects of vitamin E. Korean J Intern Med 2015;30:571–9. doi: http://dx.doi.org/10.3904/kjim.2015.30.5.571.

Boroushaki MT, Arshadi D, Jalili-Rasti H, Asadpour E, Hosseini A. Protective effect of pomegranate seed oil against acute toxicity of diazinon in rat kidney. Iran J Pharm Res 2013;12: 821–7. doi: 10.22037/ijpr.2013.1402.

Al-Attar AM, Alrobai AA, Almalki DA. Protective effect of olive and juniper leaves extracts on nephrotoxicity induced by thioacetamide in male mice. Saudi J Biol Sci 2017; 24:15–22. doi: https://doi.org/10.1016/j.sjbs.2015.08.013.

Widyastuti DA, Ristianti MA, Sari IM. The study of blood creatinin and urea concentration of Wistar rats (Rattus norvegicus) due to sodium nitrite induction. J Ilmu Kefarmasian Indones 2019;17:14-20. doi: 10.35814/jifi.v17i1.560.