Effect of Physalis angulata leaf extract cream on Interleukin-4, Interleukin-6, and Immunoglobulin-E in mice with induced atopic dermatitis
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Abstract
Background
The prevalence of atopic dermatitis (AD) and allergic or irritant contact dermatitis has been increasing significantly in the general population. Interleukin- 4 (IL-4), interleukin-6 (IL-6), and immunoglobulin E (IgE) play a key role in the pathogenesis of AD. Physalis angulata (PA) leaves reportedly have anti-inflammatory effects by impeding IL-4, IL-6, and IgE. This study aimed to evaluate the effect of PA leaf extract cream on IL-4, IL-6, and IgE using 2,4-dinitrochlorobenzene (DNCB) to induce AD-like skin inflammation in a mice model.
Methods
This study used an experimental design involving 30 BALB/c mice, that were randomized into 3 groups: 1) control group receiving no treatment; 2) Vehicle treatment group receiving vehicle cream preparation; 3) PA treatment group receiving 10% PA leaf extract cream after induction of AD-like skin inflammation by DNCB. After 30 days, tissue samples were extracted from the skin lesions to measure IL-4 and IL-6 levels, and serum to measure IgE using ELISA. One-way Anova, Kruskal-Wallis and Mann-Whitney tests were used to analyze the data.
Results
Group 3 (PA treatment) had significantly lower IL-4 (281.15 ± 43.14 pg/mL) than group 2 (vehicle cream treatment) (388.89±135.88 pg/ml) (p=0.001). However, although IL-6 and IgE levels were lower in group 3 than in group 2, the differences were statistically not significant (p=0.096 and p=0.479 respectively).
Conclusion
There were lower levels of IL-4, IL-6, and IgE in the group receiving PA leaf extract cream than in the group receiving vehicle cream preparation. Therefore, PA leaf extract cream may have therapeutic potential in AD.
Article Details
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