pISSN 1907-3062 | eISSN 2407-2230 | Universa Medicina content is searchable on DOAJ, Google Scholar, and OAI

High MMP-9 and TNF-α expression increase in preterm premature rupture of membranes

Sri Sulistyowati, Yuniarsih Zakia, Soetrisno Khasan
Submission date: Thursday, 04 February 2016
Published date: Monday, 09 May 2016
DOI: http://dx.doi.org/10.18051/UnivMed.2016.v35.33-39

Article Metrics

Abstract viewed: 399 times

Abstract


Preterm delivery is one of the causes of high perinatal morbidity and mortality. Matrix metalloproteinase 9 (MMP-9) is important for extracellular matrix (ECM) remodeling and may cause preterm labor and premature rupture of membranes (PROM). Tumor necrosis factor-α (TNF-α) as a pro-inflammatory cytokine plays a role in stimulating uterine activity and cervical ripening by degrading the ECM of the amniotic membranes through MMP-9. This study aimed to determine differences between MMP-9 and TNF-α expression of the membranes in preterm delivery with premature rupture of membranes (PPROM) and without PROM.

Methods

An analytic observational study with cross-sectional approach was conducted in 24 subjects, who were divided into 2 groups, with 12 subjects in the preterm delivery group with PROM and 12 subjects in the preterm delivery group without PROM. The expression of MMP-9 and TNF-α in the amniotic membrane was determined by immunohistochemistry. Data were analyzed using the t test.

Results

MMP-9 expression in the amniotic membrane of preterm delivery subjects with PROM (8.6 ± 3.1%/field) differed significantly with that of preterm delivery subjects without PROM (5.5 ± 2.3 %/ field) (p=0.001). TNF-α expression in the amniotic membrane of preterm delivery subjects with PROM (8.0 ±3.0%/field) also differed significantly with that of preterm delivery subjects without PROM (3,3 ± 1.5%/field) (p=0.000).

Conclusion

Expression of MMP-9 and TNF-α was higher in the amniotic membrane of preterm delivery subjects with PROM than in preterm delivery subjects without PROM and can thus be used as predictor to avoid PPROM.

Keywords


MMP-9, TNF-α; preterm delivery; premature rupture of membranes

Full Text:

PDF

References


Okeke TC, Enwereji JO, Okoro OS, Adiri CO, Ezugwu EC, Agu PU. The incidence and management outcome of preterm premature rupture of membranes (PPROM) in a tertiary hospital in Nigeria. Am J Clin Med Res 2014;2:14 – 7.

Martin JA, Hamilton BE, Sutton PD, et al. Births: final data for 2005. Natl Vital Stat Rep 2007;5;1-103.

Spong CY. Prediction and prevention of recurrent spontaneous preterm birth. Obstet Gynecol 2007;110 2 Pt 1:405–15.

Sudarmi. Hubungan ketuban pecah dini > 12 jam dengan gawat janin di ruang bersalin RSUP NTB tahun 2012. Media Bina Ilmiah 2013;7:31 – 4

Razzak MSA, Al-Sa’adi MAK, Hussainy TAIA. The role of tumor necrosis factor-alpha (TNF-Α) in the induction of preterm labor. Karbala J Med 2010;3:779 – 83.

Velemínský M, Sák P. Management of pregnancy with premature rupture of membranes (PROM). J Health Sci Management Public Health 2006:192–7.

Wiradharma, Kardana I, Wyn DAI. Risiko asfiksia pada ketuban pecah dini di RSUP Sanglah. Sari Pediatri 2013;14:316-9

Wibowo AP, Sulistyowati S, Respati SH. Difference of serum MMP-9 and TNF-α level in preterm and term premature rupture of membranes. IJOG 2015;3:15 – 8.

Weiss A, Goldman S, Shalev E. The matrix metalloproteinases (MMPS) in the decidua and fetal membranes. Front Biosci 2007;1:649 – 59.

Yoon BH, Oh SY, Romero R, et al. An elevated amniotic fluid matrix metalloproteinase-8 level at the time of mid-trimester genetic amniocentesis is a risk factor for spontaneous preterm delivery. Am J Obstet Gynecol 2001;185:1162-7.

Haider SM, Knofler. Human tumour necrosis factor: physiological and pathological roles in placenta and endometrium. Placenta 2009;30:111 – 23.

Calleja-Agius J, Muttukrishna S, Jauniaux E. The role of tumor necrosis factor-receptors in pregnancy with normal and adverse outcome. Int J Interferon, Cytokine Mediator Res 2012;4:1 – 15.

Thomakos N, Daskalakis G, Papapanagiotou A, Papantoniou N, Mesogitis S, Antsaklis A. Amniotic fluid interleukin-6 and tumor necrosis factor-a at mid-trimester genetic amniocentesis: relationship to intra-amniotic microbial invasion and preterm delivery. Eur J Obstet Gynecol Reprod Biol 2010;148:147 – 51.

Hulley SB, Cummings SR, Browner WS, Grady DG, Newman TB. Designing clinical research. 4th ed. New York : Lippincot Williams & Wilkins, Wolters Kluwer;2013.

Izumi-Yoned N, Toda A, Okabe M, Koike C, Takashima S, Yoshida T, Konishi I, et al. Alpha 1 antitrypsin activity is decreased in human amnion in premature rupture of the fetal membranes. Mol Hum Reprod 2009;15:49-57.

Sorokin Y, Romero R, Mele L, et al. Maternal serum interleukin-6, C-reactive protein, and matrix metalloproteinase-9 concentrations as risk factors for preterm birth <32 weeks and adverse neonatal outcomes. Am J Perinatol 2010;27: 631–40.

Sabarudin U, Mose JC, Krisnadi SR. Polimorfisme gen MMP-9, ekspresi MMP-9, dan indeks apoptosis sel serviks pada kehamilan 21 – 36 minggu. MKB. 2011;43:199 – 206.

Kumar D, Schatz F, Moore RM, et al. The effects of thrombin and cytokines upon the biomechanics and remodeling of isolated amnion. In Vitro Placenta 2011;32:206-13.


Refbacks

  • There are currently no refbacks.


Copyright (c) 2016 Sri Sulistyowati, Yuniarsih Zakia, Soetrisno Khasan

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Creative Commons License
Universa Medicina by Faculty of Medicine, Trisakti University is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Based on a work at https://univmed.org/ejurnal/index.php/medicina/