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Lead is able to pass through the placental barrier and interfere with fetal development. Red fruit has high antioxidant activity, due to carotenoids (pro-vitamin A), tocopherols (vitamin E) and unsaturated fatty acids. This study aims to examine the effect of red fruit oil towards placenta in pregnant mice before lead exposure.
This was a laboratory experimental post test only study, using 20 pregnant mice (Mus musculus) that were randomized into five groups. The negative control group was not exposed to lead; intervention group 0 (P0) was exposed to lead at 0.011 mg/20 g BW/day on days 6-15 of gestation. The intervention groups P1-P3 were given red fruit orally at 0.3 mL/20 g BW/day, 0.8 mL/20 g BW/day, 0.9 mL/20 g BW/day, respectively, before lead exposure. Normal trophoblast cell count and caspase-9 expression of trophoblasts were calculated. One-way ANOVA, Kruskal-Wallis and Mann Whitney tests were used to analyze the data.
The intervention groups P2 and P3 given red fruit at 0.8 mL/20 g BW/day and 0.9 mL/20 g BW/day showed a significant increase in normal trophoblast cell count of 88.2% (p=0.024) and a decrease in caspase-9 expression of 70.8% compared with P0 to which was not given red fruit (p=0.020).
Red fruit increased normal trophoblast cell count by 88.2% and decreased caspase-9 expression by 70.8% in pregnant mice before lead exposure. It should be noted that red fuit may prevent oxidative damage to cells.
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Barness EG. Teratogenic causes of malformations. Ann Clin Lab Sci 2010;40:99-114.
Garcia-Nìno WR, Chaverri JP. Protective effect of curcumin against heavy metals-induced liver damage. Food Chem Toxicol 2014;69:182–201.
Levy R, Smith SD, Yusuf K, et al. Trophoblast apoptosis from pregnancies complicated by fetal growth restriction is associated with enhanced p53 expression. Am J Obstet Gynecol 2002;186: 1056-61.
Sarungallo ZL, Hariyadi P, Andarwulan N, et al. Analysis of á-cryptoxanthin, b-cryptoxanthin, á-carotene, and â-carotene of Pandanus conoideus oil by high-performance liquid chromatography (HPLC). Procedia Food Sci 2015;3:231–3.
Dearth RK, Hiney JK, Srivastava V, et al. Effects of lead (Pb) exposure during gestation and lactation on female pubertal development in the rat. Reprod Toxicol 2002;16:343–53.
Isradji I. Pengaruh pemberian Pb-asetat terhadap fertilitas mencit jantan, dimonitor melalui jumlah kebuntingan dan jumlah anak sekelahiran. Sains Medika 2010;2:2:170-177.
Sharma RN, Qureshi SM, Panwar K. Lead induced infertility in Swiss mice and role of antioxidants. Universal J Environ Res Technol 2012;2:72-82.
Rohman A, Riyanto S, Yuniarti N, et al. Antioxidant activity, total phenolic, and total flavaonoid of extracts and fractions of red fruit (Pandanus conoideus Lam). Int Food Res J 2010; 17:97-106.
Widjiati AWR, Arifin MZ. Pengaruh berbagai konsentrasi krioprotektan propanediol pada proses vitrifikasi terhadap viabilitas embrio mencit pasca thawing. Veterinaria Medika 2011; 4:2.
Aprioku JS, Siminialayi IM. Maternal lead exposure and pregnancy outcome in Wistar albino rats. J Toxicol Environ Health Sci 2013; 5:185-93.
Udju IST, Martono Y, Soetjipto H. Ekstrak enzimatis antioksidan buah merah (Pandanus conoideus Lam.) sebagai antikolesterol terhadap mencit putih jantan galur swiss. Prosiding Seminar Nasional Sains dan Pendidikan Sains VII UKSW. Salatiga;2012.p.117-26.
Nowak M, Madej JA, Dziegeil P. Intensity of Cox2 expression in cells of soft tissue fibrosarcomas in dogs as related to grade of tumour malignancy. Bull Vet Inst Pulawy 2007; 51:275-9.
Suryohudoyo P. Kapita selekta ilmu kedokteran molekuler. Jakarta: Sagung Seto;2007.
McIlwain DR, Berger T, Mak TW. Caspase functions in cell death and disease. Cold Spring Harb Perspect Biol 2013;5:a008656.
Halperin R, Peller S, Sandbank J, et al. Expression of the p53 gene and apoptosis in gestational tropoblastic disease. Placenta 2000; 21:58–62.
Qumsiyeh MB, Kim KR, Ahmed MN, et al. Cytogenetics and mechanisms of spontaneous abortions: increased apoptosis and decreased cell proliferation in chromosomally abnormal villi. Cytogenet Cell Genet 2000;88:230-5.
Veras MM, Damaceno-Rodrigues NR, Caldini EG, et al. Particulate urban air pollution affects the functional morphology of mouse placenta. Biol Reprod 2008;79:578-84.
Christijanti W, Utami NR, Iswara A. Pemberian antioksidan vitamin C dan E terhadap kualitas spermatozoa tikus putih terpapar allethrin. Biosaintifika 2010;2:18-26.
Vaya J, Aviram M. Nutritional antioxidants: mechanisms of action, analyses of activities and medical applications. Curr Med Chem Imm Endo Metab Agents 2001;1.
Sajitha GR, Jose R, Andrews A, et al. Garlic oil and vitamin E prevent the adverse effects of lead acetate and ethanol separately as well as in combination in the drinking water of rats. Indian Clin Biochem 2010;25:280-8.
Sharma B, Singh S, Siddiqi NJ. Biomedical implications of heavy metals induced imbalances in redox systems. Bio Med Res Int 2014, Article ID 640754, 26 pages http://dx.doi.org/10.1155/2014/640754.
Polyakov NE, Foscan AL, Bowman MK, et al. Free radical formation in novel carotenoid metal ion complexes with astaxanthin. J Phys Chem B 2010;114:16968–77.