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Stress is one of the factors that cause apoptosis in neuronal cells. Centella
asiatica has a neuroprotective effect that can inhibit apoptosis. This study
aimed to examine the effect of Centella asiatica ethanol extract on B-cell
lymphoma 2 (Bcl-2) protein expression in the prefrontal cortex of rats.
An experimental study was conducted on 34 brain tissue samples from male
Sprague Dawley rats exposed to chronic restraint stress for 21 days. The
samples were taken from following groups: non-stress group K, negative
control group P1 (stress + arabic gum powder), P2 (stress + C.asiatica at
150 mg/kgBW), P3 (stress + C.asiatica at 300 mg/kg BW), P4 (stress +
C.asiatica at 600 mg/kg body weight) and positive control group P5 (stress
+ fluoxetine at 10 mg/kgBW). The samples were made into sections that
were stained immunohistochemically using Bcl-2 antibody to determine the
percentage of cells expressing Bcl-2. Data were analyzed using one way
ANOVA test followed by a post - hoc test.
There were significant differences in mean Bcl-2 expression between the
groups receiving Centella asiatica compared with the non-stress group and
stress-only group (negative control group) (p<0.05). The results were
comparable to those of the fluoxetine treatment group.
The Centella asiatica ethanol extract was able to increase Bcl-2 expression
in the prefrontal cortex of Sprague Dawley rats exposed to restraint stress.
This study suggests that Centella asiatica may be useful in the treatment of
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