Assessment of microarchitecture and crystal structure of hydroxyapatite in osteoporosis

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Published: 2011-04-23
DOI: https://doi.org/10.18051/UnivMed.2011.v30.29-35
Keywords:
Microarchitecture, hydroxyapatite, osteoporosis

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Zairin Noor
Department of Orthopedics, Ulin Regional General Hospital, Faculty of Medicine, Lambung Mangkurat University, Banjarmasin
Sutiman B Sumitro
Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang
Mohammad Hidayat
Department of Orthopedics, Syaiful Anwar Regional General Hospital, Faculty of Medicine, Brawijaya University, Malang
Agus Hadian Rahim
Department of Orthopedics, Hasan Sadikin Hospital, Faculty of Medicine, Padjadjaran University, Bandung
Ahmad Taufiq
X-ray Diffraction Laboratory, Central Laboratory, Faculty of Mathematics and Natural Sciences, Malang State University

Abstract

Osteoporosis is characterized by lower bone mineral density (BMD) and microarchitectural degeneration, which tends to increase bone fragility and fracture risk. Bone microstructure depends on interactions between the mineral atoms, which may perform substitution or incorporation into bone crystals, and may dynamically take over the function of calcium or may become a complementary part. The mineral atoms may also become a composite in the hydroxyapatite crystals. The aim of this study was to find an association between the bone microstructure and hydoxyapatite crystal structure in osteoporosis, in comparison to normal bone. The subjects of this study were surgery patients at the Department of Orthopedics of Ulin General Hospital in Banjarmasin and other centers. Inclusion criteria consisted of the presence of fracture of trabecular bone, normal or osteoporotic BMD values, and no past history of chronic disease. Bone was obtained from fracture patients during surgery. The characteristics of the hydroxyapatite crystals were analyzed by X-ray diffraction (XRD) and the microarchitecture by scanning electron microscopy (SEM). SEM showed degeneration of the microarchitecture of osteoporotic bone, in comparison with normal bone. On XRD there was a peak of hydoxyapatite crystals only and no other crystal phases. Diffraction patterns showed a larger crystal size in osteoporotic bone as compared to normal bone, indicating higher porosity. It may be concluded that there is a difference in crystal size and morphologic distribution of hydoxyapatite in osteoporotic bone, determining bone microarchitecture.

Article Details

Issue

Vol. 30 No. 1 (2011)

Section

Review Article

The journal allows the authors to hold the copyright without restrictions and allow the authors to retain publishing rights without restrictions.

How to Cite

Assessment of microarchitecture and crystal structure of hydroxyapatite in osteoporosis. (2011). Universa Medicina, 30(1), 29-35. https://doi.org/10.18051/UnivMed.2011.v30.29-35

References

Huang Q, Kung AWC. Genetics of osteoporosis. Mol Genet Metabol 2006;88:295-306.

Duncan EL, Brown MA. Genetic studies in osteoporosis – the end of the beginning. Arthritis Res Ther 2008;10:214.

Brandao CMR, Lima MG, da Silva AL, Silva GD, Guerra AA Jr, Acurcio FA. Treatment of postmenopausal osteoporosis in women: a systematic review. Cad Saude Publica 2008;24 Supl 4:S592-606.

Handa R, Kalla AA, Maalouf G. Osteoporosis in developing countries. Best Practice Pract Res Clin Rheumat 2008;22:693-708.

Cheng XG, Yang DZ, Zhou Q. Age-related bone mineral density, bone loss rate, prevalence of osteoporosis, and reference database of women at multiple centers in China. J Clin Densitom 2007;6:276-84.

Zhang ZL, Qin YJ, Huang QR. Bone mineral density of the spine and femur in healthy Chinese men. Asian J Androl 2006;8:419-27.

Lynn HS, Lau EM, Au B, Leung PC. Bone mineral density reference norms for Hong Kong Chinese. Osteoporos Int 2005;16:1663-8.

Sadat-Ali M, Al-Elq A. Osteoporosis among male Saudi Arabs: a pilot study. Ann Saudi Med 2006;26:450-4.

El-Desouki MI, Sulimani RA. High prevalence of osteoporosis in Saudi men. Saudi Med J 2007;28:774-7.

PEROSI. Indonesian osteoporosis: fact, figures, and hopes. Indonesian Osteoporosis Association, 2009.

Prihartini S. Faktor determinan risiko osteoporosis. Bogor: Pusat Penelitian Gizi dan Makanan Departemen Kesehatan;2009.

Prentice A, Jarjou LAM, Cole TJ, Stirling DM, Dibba B, Fairweather TS. Calcium requirements of lactating Gambian mothers: effect of calcium supplement on breast milk calcium concentration, maternal bone mineral content, and urinary calcium excretion. Am J Clin Nutr 1995;62:58-67.

Cumming SR, Melton LJ III. Epidemiology and outcome of osteoporosis fractures. Lancet 2002; 359:1761-7.

Leventouri T. Synthetic and biological hydroxyapatites: crystal structure questions. Biomaterials 2006;27:3339–42.

Dilworth L, Omoruyi FO, Reid W, Asemota HN. Bone and faecal minerals and scanning electron microscopic assessments of femur in rats fed phytic acid extract from sweet potato (Ipomoea batatas). Biomaterials 2008;21:133-41.

Frasca P, Harper RA, Katz JL. Scanning electron microscopy studies of collagen, mineral and ground substance in human cortical bone. Scanning Electron Microscope 1981:339-46.

Braidotti P, Branca FP, Stagni L. Scanning electron microscopy of human cortical bone failure surfaces. J Biomech 1997;30:155-62.

Shen Y, Zhang Z, Jiang S, Jiang L, Dai L. Postmenopausal women with osteoarthritis and osteoporosis show different ultrastructural characteristics of trabecular bone of the femoral head. BMC Musculoskeletal Disorders 2009;10:35 doi:10.1186/1471-2474-10-35.

Ren F, Xin R, Ge X, Leng Y. Characterization and structural analysis of zinc-substituted hydroxyapatites. Acta Biomaterialia 2009; 5:3141-9.

Miyaji F, Kono Y, Suyama Y. Formation and structure of zinc-substituted calcium hydroxyapatite. Mater Res Bull 2005;40:209–20.

Li MO, Xiao X, Liu R, Chen C, Huang L. Structural characterization of zinc-substituted hydroxyapatite prepared by hydrothermal method. J Mater Sci Mater Med 2008;19:797-803.

Mikrajudin A, Khairurrijal. Derivation of Scherrer relation using an approach in basic physics course. J Nanosains Nanotechnol 2008;1:45-51.

Zioupos P, Hansen U, Currey JD. Microcracking damage and the fracture process in relation to strain rate in human cortical bone tensile failure. J Biomech 2008;41:2932-9.

Vallet-Regi M, Arcos D. Biomimetic nanoceramics in clinical use: from materials to applications. Cambridge: The Royal Society of Chemistry Publishing;2008.