Dendrophthoe pentandra methanolic leaf extract increases progesterone levels in female rats

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Lazuardi Mochamad
Bambang Hermanto


Human infertiliy cases in Indonesia have tended to increase at about 2-5%
annually since 2000. Many tropical plants in Indonesia are potential sources of novel anti-infertility compounds, e.g. Dendrophthoe pentandra L. Miq. (benalu duku), a parasitic plant growing on Lansium domesticum. The objective of this study was to identify the effect of crude methanolic Dendrophthoe pentandra leaf extract on follicle stimulating hormone (FSH) and progesterone levels in female rats.
Fourteen Rattus norvegicus Wistar strain female rats were divided into an
intervention group and a control group, and synchronized to estrus via the
pheromone synchronizing method. The intervention group was given daily single intramuscular injections of crude methanolic Dendrophthoe pentandra leaf extract at 100 mg/kgBW for 4 days, while the control group was given daily single intramuscular injections of 1 mL of distilled water. Determination of FSH and progesterone levels in whole blood was done using the Randox Evidence Investigator analyzer. The data were subjected to the t test for two independent samples.
Mean FSH in the intervention group was 9.28 ± 6.72 mIU/mL, which was lower than mean FSH of 24.80 ±16.35 mIU/mL in controls (p<0.05). Mean progesterone level in the intervention group was 33.55±13.96 nmol/L, twice as high as that in the control group, which was 18.47± 06.47 nmol/L (p<0.05).
Crude methanolic Dendrophthoe pentandra leaf extract reduces FSH and
increases progesterone levels in female rats, but cannot yet be recommended for use as fertility hormone inhibitor or stimulator in rats.

Article Details

How to Cite
Mochamad, L., & Hermanto, B. (2014). Dendrophthoe pentandra methanolic leaf extract increases progesterone levels in female rats. Universa Medicina, 33(2), 100–108.
Review Article


Bashin S, Cunningham GR, Hayes FJ, Matsumoto AM, Snyder PJ, Swerdloff RS, et al. Testosterone therapy in adult men with androgen deficiency syndromes: an Endocrine Society clinical practice guideline. J Clin Endocrinol Metab 2010;95:2536-59.

Onyeka CA, Aligwekwe AU, Olawuyi TS, Nwakanma AA, Kalu EC, Oyeyemi AW. Antifertility effects of ethanolic root bark extract of Chrysophyllum albidum in male albino rats. Int J Appl Res Nat Prod 2012;5:12-7. 3. Pathak AK, Mallurwar VR, Kondalkar AK, Soni S. A review of plants with anti-fertility activity. Nig J Nat Prod Med 2005;9:4-10.

Lazuardi M, Nuraini F, Ratna SM, Roostantia I. Screening of benalu duku for antiproliferation of myeloma. In: Puji S, Mustopha BA, Yunus M, Suzanita U, Suwarno, editors. Strategies for the control prevention of zoonotic diseases. Surabaya: Airlangga University Press;2010.p.65-8.

Lazuardi M. The overview of Benalu duku (Dendrophthoe spec.,) as a parasites herbal medicine with potential for treatment Trypanosomiasis. In: Endang E, Erni, editors.

Abstract and Programs Pertemuan Ilmiah Tahunan Nasional I Perhimpunan Farmasi Kedokteran Indonesia (PEFARDI) 7-8 Mei 2011. Bandung: Bagian Farmasi Kedokteran Universitas Maranatha. 2011.p.15-20.

Zaimah I. Skrining fitokimia ekstrak herbal Benalu duku (Dendrophthoepetandra L. Miq.,) dan uji aktivasi terhadap larva udang (Artemia salina Leach) dengan metode BSLT (brine shrimp lethality test) [Skripsi]. Solo: Fakultas Sains dan

Teknologi (FST) Universitas Jenderal Soedirman;2011.

Nina A, Taufik F, Akhmad D. Bioactivities evaluation of Indonesian mistletoes 108 (Dendrophthoe pentandra (L.) Miq.) leaves extracts. J Appl Pharm Sci 2012;1:24-7.

Lazuardi M, Bambang H. Assesment of luteinizing testosterone prolactin estrogen hormone after giving crude methanol extract of benalu duku leaves on healthy adult female rat. In: Lazuardi M, Rinidar, Ietje W, editors. Proceeding 2nd Congress of Indonesia Veterinary Pharmacy and Pharmacology Association. 21-22 Sept 2013. Airlangga University Press; 2013.p.28-9.

Thomson RN, Taha M, Napier A, Welkesa KS. The induction of pregnancy block in mice by bodily fluids via the vomeronasal organ. Biochem Physiol 2013;2:2-5.

Saadat P, Latiffah AL, Sabariah AR, Mohammad AD, Hanachi P. Assessing estrogenic activity of Nigella sativa in ovariectomized rats using vaginal cornification assay. African J Pharm Pharmacol 2011;5:137-42.

Randox Laboratories Ltd. Fertility Hormone Array (FERT) Crumlin, County Antrim UK;2013.

Izuchukwu SO, Chike FO, Chinaza CO. The effect of increasing number of strange male mice on Bruce effect. Veterinarskhi Arhiv 2012;82:103-14.

Cornelius PCP. Study of effect of sweet basil (Ocimum basilicum Linn) essential oil of various dosages on estrus cycle of Sprague-Dawley rats. J Sains Vet 2012;30:87-96.

Tjuk IR. Profile of insulin-like growth factor-I (IGFI) in pregnant thoroughbred mare serum. In: Lazuardi M, Rinidar, Ietje W, Editors. Proceeding 2nd Congress of Indonesia Veterinary Pharmacy and Pharmacology Association. 21-22 Sept 2013. Airlangga University Press;2013.p.43-8.

Briggs J. Alliance for contraception in cats and dogs.USA:ACC & D;2013.

Otto JR, Freeman MJ, Malau-Aduli BS, Nichols PD, Lane PA, Malau-Aduli EO. Reproduction and fertility parameters of dairy cows supplemented with omega-3 fatty acid-rich canola oil. Ann Res Rev Biol 2014;4:1611-36.

Konishi S1, Brindle E, Guyton A, O’Connor KA. Salivary concentration of progesterone and cortisol significantly differs across individuals after correcting for blood hormone values. Am J Phys Anthropol 2012;149:231-41.

Chang H1, Wan Y, Wu S, Fan Z, Hu J. Occurrence of androgens and progestogens in wastewater treatment plants and receiving river waters: comparison to estrogens. Water Res 2011;45:732-40.

Ernst M1, de Padua RM, Herl V, Muller-Uri F, Kreis W. Expression of 3beta-HSD and P5betaR, genes respectively coding for delta5-3betahydroxysteroid dehydrogenase and progesterone 5beta-reductase, in leaves and cell cultures of

Digitalis lanata EHRH. Planta Med 2010;76:923-7.

Gupta RS1, Kachhawa JB. Evaluation of contraceptive activity of methanol extract of Dendrophthoe falcata stem in male albino rats. J Ethnopharmacol 2007;30:215-8.

Pooja S, Irchhaiya R, Bhawna S, Gayatri S, Santosh K. Anticonvulsant and muscle relaxant activity of the ethanolic extract of stems of Dendrophthoe falcata (Linn. F.) in mice. Indian J Pharmacol 2011;43:710-3.