Stenochlaena palustris aqueous extract reduces hepatic peroxidative stress in Marmota caligata with induced fever

Main Article Content

Adenan Adenan
Eko Suhartono


The kelakai fern (Stenochlaena palustris) contains bioactive substances, such as flavonoids, steroids, and alkaloids. The kelakai is used by the Kenyah Dayak communities for the treatment of anemia, fever, and cutaneous disorders. Fever is increased temperature regulation of the body, which process indirectly increases the production of peroxides, leading to peroxidative stress. The aim of this research study was to evaluate the influence of an aqueous extract of the kelakai fern on peroxidative stress in the hoary marmot (Marmota caligata) with experimentally-induced fever. This was a true experimental study, with post test-only and control group design, involving 6 groups, each made up of 4 animals. Peroxide concentration was measured by a modification of the FOX2 method and peroxidase activity by the Kanehira method. The kelakai extract was administered to the animals by gavage at weight-adjusted dosages between 7.75 x 10-4 mg/kg body weight to 1.24 x 10-2 mg/kg body weight. Data were analyzed using singe linear regression test. The results showed that kelakai extract dose significantly reduced the peroxide levels, regression equation with the Y = 2.513-106.03 X (R2 = 0.8057), indicating that 80.5% variation of peroxide levels is predicted by the kelakai extract dose. From the results we conclude that kelakai aqueous extract decreases peroxide levels in hoary marmots with experimentally-induced fever.

Article Details

How to Cite
Adenan, A., & Suhartono, E. (2016). Stenochlaena palustris aqueous extract reduces hepatic peroxidative stress in Marmota caligata with induced fever. Universa Medicina, 29(3), 123–128.
Review Article


Suhartono E, Yasmina A. Kajian farmakologis ekstrak air tumbuhan kelakai sebagai antiyeri dan antiradang dengan pendekatan mekanisme oksidatif. Laporan Penelitian Universitas Lambung Mangkurat; 2007.

Maharani, Haidah, Hainiyah. Studi potensi kelakai sebagai pangan fungsional. Kumpulan Makalah PIMNAS XIX, 26-29 Juli 2006;Universitas Muhamadiyah Malang; 2006.

Suhartono E. Potention of aquaeus extract kelakai as antiinflammation by oxidative mechanism, Congress International Korean Medicine; 2008 October 22-23;Sangji University Korea; 2008.

Suhartono E, Bakhriansyah M, Sandi DA, Prabandari K. Hydrogen peroxide concentration and catalase activity in the plasma of fever Marmota caligata after giving extracts of Stenochlaena palustris. Proceedings of the 38th Meeting of National Working Group for Indonesian Medicinal Plant; 2010 July 23-24; Widya Mandala University; 2010.

Kanehira T, Shibata K, Kashiwazaki H, Inoue N, Morita M. Comparison of antioxidant enzymes in saliva of elderly smokers and non-smokers. Gerodontology 2006;23:38-42.

Devaux Y, Seguin C, Grosjean S, Talance N, Camaeti V, Burlet A, et al. Lipopolysaccharide-induced increase of prostaglandin E2 is mediated by inducible nitric oxide synthase activation of the constitutive and induction of membrane-associated prostaglandin E synthase. J Immunol 2001;167:3962-71.

Warner TD, Vojnovic I, Giuliano F, Jime’nez R, Bailey DB, Mitchell JA. Cyclooxygenase 1, 2, and 3 and the production of prostaglandin I2: Investigating the activities of acetaminophen and cyclooxygenase-2-selective inhibitors in rat tissues. JPET 2004;310: 642-47.

Lobanova EM, Tahanovich AD. Hydrogen peroxide metabolism in alveolar macrophages after exposure to hypoxia and heat. Physiol. Res. 2006;55:569-75.

Guzik TJ, Korbut R, Adamek-Guzik T. Nitric oxide and superoxide in inflammation and immune regulation. J Physiol Pharmacol 2003;54:469-87.

Matsumura KI, Matsumura K, Terao A, Watanabe Y. Attenuated fever in pregnant rats is associated with blunted synthesies of brain cyclooxygenase-2 and PGE2. Am J Physiol Regul Integr Comp Physiol 2002;283:R1346-53.

Byrne, DJO., Devaraj. S., Grundy, SM., Jialal I. Comparison of the antioxidant effects of concord grape juice flavonoids and á-tocopherol on markers of oxidative stress in healthy adults. Am J Clin Nutr 2002;76:1367–74.

Pignatelli P, Pulcinelli FM, Celestini A, Lenti L, Ghiselli A, Gazzaniga PP, et al. The flavonoids quercetin and catechin synergistically inhibit platelet function by antagonizing the intracellular production of hydrogen peroxide. Am J Clin Nutr 2000;72:1150-5.

Patral A, Jha S, Murthy PN, Chattopadhyay P, Panigrahi G, Roy D, et al. Anti-Inflammatory and antipyretic activities of Hygrophila spinosa T. Anders leaves (Acanthaceae). Trop J Pharm Res 2009;8:133-7.

Ranju SP, Ariharasivakumar G, Kundlik G, Ashutosh U. In-vitro antioxidative activity of phenolic and flavonoid compounds extracted from seeds of Abrus precatorius. Int J Pharmacy Pharmaceutical Sciences 2009:1:136-40

O’byrne DJ, Devaraj S, Grundy SM, Jialal I. Comparison of the antioxidant effects of Concord grape juice flavanoid and á-tocopherol on markers of oxidative stress in healthy adults. Am J Clin Nutr 2002;76:1367-74.

Konyahoglu S, Karamenderes C. Screening of total flavanoid, phenol contents and antioxidant capacities of some Achillea L. Species growing in Turkey. Acta Pharmaceutica Turcica 2004;46:163-70.

Sudjarwo SA. The potency of piperine as antiinflammatory and analgesic in rats and mice. Folia Medica Indonesiana 2006;41:190-4.

Teh BS, Seow WK, Li SY, Thong YH. Inhibition of prostaglandin and leukotrien generation by the plant alkaloids tetradine and barbamine. J Immunopharmacol 2000;12:321-6.

Moura DJ, Marc FR, Jane MB, Joao APH, Jenifer S. Antioxidant properties of b-carboline alkaloids are related to their antimutagenic and antigenotoxic activities. Mutagenesis 2007;22: 293–302.

Runtti PP, Lakari E, Raivio KO, Kinnula VL. Expression of antioxidant enzymes in human inflammatory cells. Am J Physiol Cell Physiol 2000;278:118–25.