Dengue virus transovarial transmission by Aedes aegypti

Main Article Content

Monica Dwi Hartanti
Suryani Suryani
Ingrid A. Tirtadjaja


Dengue is a disease that is caused by dengue virus and transmitted to humans through the bite of infected Aedes mosquitoes, especially Aedes aegypti. The disease is hyper-endemic in Southeast Asia, where a more severe form, dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS), is a major public health concern. The purpose of the present study was to find evidence of dengue virus transovarial transmision in local vectors in Jakarta. Fifteen Aedes larvae were collected in 2009 from two areas in Tebet subdistrict in South Jakarta, namely one area with the highest and one with the lowest DHF prevalence. All mosquitoes were reared inside two cages in the laboratory, eight mosquitoes in one cage and seven mosquitoes in another cage and given only sucrose solution as their food. The results showed that 20% of the mosquitoes were positive for dengue virus. Dengue virus detection with an immunohistochemical method demonstrated the occurrence of transovarial transmission in local DHF vectors in Tebet subdistrict. Transovarial dengue infection in Ae.aegypti larvae appeared to maintain or enhance epidemics. Further research is needed to investigate the relation of dengue virus transovarial transmission with DHF endemicity in Jakarta.

Article Details

How to Cite
Hartanti, M. D., Suryani, S., & Tirtadjaja, I. A. (2010). Dengue virus transovarial transmission by Aedes aegypti. Universa Medicina, 29(2), 65–70.
Review Article


Clarke T. Dengue virus: break-bone fever. Nature 2002;416:672–4.

World Health Organization. Dengue fever in Indonesia – update 3. Communicable Disease Surveillance & Response (CSR). Geneva : World Health Organization; 2004.

Joshi V, Mourya DT, Sharma RC. Persistence of dengue-3 virus through transovarial transmission passage in successive generations of Aedes aegypti mosquitoes. Am J Trop Med Hyg 2002; 67:158–61.

Gubler DJ. The global emergence/resurgence of arboviral diseases as public health problems. Arch Med Res 2002;33:330–42.

Borucki MK, Kempf BJ, Blitvich BJ, Blair CD, Beaty BJ. La Crosse virus: replication in vertebrate and invertebrate hosts. Microbes Infect 2002;4:341–50.

Angel B, Joshi V. Distribution and seasonality of vertically transmitted dengue viruses in Aedes mosquitoes in arid and semi-arid areas of Rajasthan, India. J Vector Borne Dis 2008;45:56–9.

Rohani A, Zamree I, Lee HL, Mustafakamal I. Detection of transovarian dengue virus for field caught Aedes aegypti and Aedes albopictus mosquito using C6/36 cell line and RT-PCR. Trop Biomed 2005;22:149–54.

Umniyati RS. Teknik Imunositokimia dengan menggunakan antibodi monoklonal DSSC7 untuk kajian patogenesis infeksi dan penularan transovarial virus dengueserta surveilansi vektor dengue [dissertation]. Fakultas Kedokteran, Universitas Gadjah Mada; 2009.

Arunachalam N, Tewari SC, Thenmozhi V, Rajendran R, Paramasivan R, Manavalan R, et al. Natural vertical transmission of dengue viruses by Aedes aegypti in Chennai, Tamil Nadu, India. Indian J Med Res 2008;127:395-7.

Günther J, Martínez-Muñoz JP, Pérez-Ishiwara DG, Salas-Benito J. Evidence of vertical transmission of dengue virus in two endemic localities in the state of Oaxaca, Mexico. Intervirology 2007;50:347-52.

Lee HL, Rohani A. Transovarial transmission of Dengue virus in Aedes aegypti and Aedes albopictus in relation to Dengue outbreak in an urban area in Malaysia. Dengue Bull 2005;29: 106-11.

Mourya DT, Gokhale, Basu A, Barde PV, Sapkal GN, Padbidri VS, et al. Horizontal and vertical transmission of dengue-2 virus in highly and lowly susceptible strains of Aedes aegypti mosquitoes. Acta Virol 2001;45: 67–71.

Perich MJ, Davila G, Turner A, Garcia A, Nelson M. Behavior of resting Aedes aegypti (culicidae: diptera) and its relation to ultra-low volume adulticide efficacy in Panama City, Panama. J Med Entomol 2000;37:541-6.

Xu G, Dong H., Shi N, Liu S, Zhou A, Cheng Z, et al. An outbreak of dengue virus serotype 1 infection in Cixi, Ningbo, People’s Republic of China, 2004. associated with a traveler from Thailand and high density of Aedes albopictus. Am J Trop Med Hyg 2007;76:1182–8.

Thavara U, Siriyasatien P, Tawatsin A, Asavadachanukorn P, Anantapreecha S, Wongwanich R, et al. Double infection of heteroserotypes of dengue viruses in field populations of Aedes aegypti and Aedes albopictus (Diptera: Culicidae) and serological features of dengue viruses found in patients in southern Thailand. Southeast Asian J Trop Med Public Health 2006;37:468-76.

Lembaga Penelitian dan Pengembangan Departemen Kesehatan. Demam berdarah dengue. Bagian Jaringan Informasi Iptek dan Promosi Penelitian, Sekretariat Badan Litbang Kesehatan, c2007. Available at: http// . Accessed June 10, 2008.

Dinas Kesehatan DKI. Demam berdarah dengue, makin mengecoh makin mengganas. Dinas Kesehatan Profinsi DKI Jakarta, c2007. Available at: http// Accessed June 10, 2008.

Akbar MR, Agoes R, Djatie T, Kodyat S. PCR detection of Dengue transovarial transmissibility in Aedes aegypti in Bandung, Indonesia. Proc ASEAN Congr Trop Med Parasitol 2008;3:84-9.