Asiaticoside induces cell proliferation and collagen synthesis in human dermal fibroblasts

Main Article Content

Linda Yuliati
Etik Mardliyati
Kusmarinah Bramono
Hans Joachim Freisleben


Asiatiocoside, a saponin component isolated from Centella asiatica can improve wound healing by promoting the proliferation of human dermal fibroblasts (HDF) and synthesis of collagen. The skin-renewing cells and type I and III collagen synthesis decrease with aging, resulting in the reduction of skin elasticity and delayed wound healing. Usage of natural active compounds from plants in wound healing should be evaluated and compared to retinoic acid as an active agent that regulates wound healing. The aim of this study was to compare and evaluate the effect of asiaticoside and retinoic acid to induce greater cell proliferation and type I and III collagen synthesis in human dermal fibroblast.

Laboratory experiments were conducted using human dermal fibroblasts (HDF) isolated from human foreskin explants. Seven passages of HDF were treated with asiaticoside and retinoic acid at several doses and incubated for 24 and 48 hours. Cell viability in all groups was tested with the MTT assay to assess HDF proliferation. Type I and III collagen synthesis was examined using the respective ELISA kits. Analysis of variance was performed to compare the treatment groups.

Asiaticoside had significantly stronger effects on HDF proliferation than retinoic acid (p<0.05). The type III collagen production was significantly greater induction with asiaticoside compared to retinoic acid (p<0.05).

Asiaticoside induces HDF proliferation and type I and III collagen synthesis in a time- and dose-dependent pattern. Asiaticoside has a similar effect as retinoic acid on type I and type III collagen synthesis.

Article Details

How to Cite
Yuliati, L., Mardliyati, E., Bramono, K., & Freisleben, H. J. (2015). Asiaticoside induces cell proliferation and collagen synthesis in human dermal fibroblasts. Universa Medicina, 34(2), 96–103.
Review Article
Author Biographies

Linda Yuliati, Tarumanegara University

Department of dermatovenereology, Faculty of Medicine

Etik Mardliyati, University of Indonesia

Centre for Pharmaceutical and Medical Technology Agency for the Assessment and Application for Technology and Higher Education

Kusmarinah Bramono, University of Indonesia

Department of Dermatovenereology, Faculty of medicine

Hans Joachim Freisleben, University of Indonesia

Biomedical Sciences Doctoral Program, Faculty of Medicine


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