Main Article Content
Asiatiocoside, a saponin component isolated from Centella asiatica can improve wound healing by promoting the proliferation of human dermal fibroblasts (HDF) and synthesis of collagen. The skin-renewing cells and type I and III collagen synthesis decrease with aging, resulting in the reduction of skin elasticity and delayed wound healing. Usage of natural active compounds from plants in wound healing should be evaluated and compared to retinoic acid as an active agent that regulates wound healing. The aim of this study was to compare and evaluate the effect of asiaticoside and retinoic acid to induce greater cell proliferation and type I and III collagen synthesis in human dermal fibroblast.
Laboratory experiments were conducted using human dermal fibroblasts (HDF) isolated from human foreskin explants. Seven passages of HDF were treated with asiaticoside and retinoic acid at several doses and incubated for 24 and 48 hours. Cell viability in all groups was tested with the MTT assay to assess HDF proliferation. Type I and III collagen synthesis was examined using the respective ELISA kits. Analysis of variance was performed to compare the treatment groups.
Asiaticoside had significantly stronger effects on HDF proliferation than retinoic acid (p<0.05). The type III collagen production was significantly greater induction with asiaticoside compared to retinoic acid (p<0.05).
Asiaticoside induces HDF proliferation and type I and III collagen synthesis in a time- and dose-dependent pattern. Asiaticoside has a similar effect as retinoic acid on type I and type III collagen synthesis.
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Davis SC, Perez R. Cosmeceutical and natural products: wound healing. Clin Dermatol 2009; 27:502-6.
Chanchal D, Swarniata S. Novel approaches in herbal cosmetics. J Cosmet Dermatol 2008;7:89-95.
Tiwari S, Geniot S, Gambhir IS. Centella Asiatica: a concise drug review with probable clinical uses. J Stress Physiol Biochem 2011;7: 39-42.
Hashim P, Sidek H, Helan MHM, et al. Triterpene composition and bioactivities of Centella asiatica. Molecules 2011;16:1310-22.
Pitella F, Dutra RC, Junior DD. Antioxidant and cytotoxic activities of Centella asiatica (L) Urb. Int J Mol Sci 2009;10:3713–21.
Paocharoen V. The efficacy and side effects of oral Centellla asiatica extract for wound healing promotion in diabetic wound patients. J Med Assoc Thai 2010;93:S166–9.
Zainol NA, Voo SC, Sarmidi MR, et al. Profiling of Centella asiatica (L.) Urban extract. Malaysian J Anal Sci 2008;12;322-7.
Gohil KJ, Patel JA, Gajjar AK. Pharmacological review on Centella asiatica: a potential herbal cure-all. Indian J Pharm Sci 2010;72:546-56.
Bhavna D, Jyoti K. Centella asiatica: the elixir of life. IJRAP 2011;2:431-8.
Lu L, Ying K, Wei S, et al. Asiaticoside induction for cell-cycle progression, proliferation and collagen synthesis in human dermal fibroblasts. Int J Dermatol 2004;43:801-7.
Loc NH, Tam An NT. Asiaticoside production from Centella (Centella asiatica L. Urban) cell culture. Biotechnol Bioprocess Engineering 2010;15:1065–70.
Lintner K, Chamberlin CM, Modun P, et al. Cosmeceuticals and active ingredients. Clin Dermatol 2009;27:461-8.
Hadi RS, Kusuma I, Sandra Y. Allogenic human dermal fibroblast are viable in peripheral blood mononuclear co-culture. Univ Med 2014;33:34-42.
Jurzak M, Latocha M, Goiniczek K. Influence of retinoids on skin fibroblasts metabolism in vitro. Acta Pol Pharm Drug Res 2008;1:85-95.
Gimeno A, Zarogoza R, Vivosese I. Retinol at concentration greater than the physiological limit, induces oxidative stress and apoptosis in human dermal fibroblast. Exp Dermatol 2004; 13:45-54.
Wu F, Bian D, Xia Y, et al. Identification of major active ingredients responsible for burn wound healing of Centella asiatica herbs. J Evid Based Complement Altern Med 2012. Article ID 848093, 13 pages. doi:10.1155/2012/848093.
Pareda MDCV, Dieamant GDC, Eberlin S, et al. Effect of green Coffea Arabica L. seed oil on extracellular matrix components and water- channel expression in in vitro and ex vivo human skin models. J Cosmetic Dermatol 2009;8:58-62.
Song J, Day Y, Bian D, et al. Madecassoside induce apoptosis of keloid fibroblast via a mitochondria–dependent pathway. Drug Dev Res 2011;72:315-22.
Ju-lin X, Shao-hai Q, Tian-zeng L, et al. Effect of asiaticoside on hypertrophic scar in the rabbit ear model. J Cutan Pathol 2009;36:234-9.
Kaji R, Kwak HSR, Schumacker WE, et al. Improvement of naturally aged skin with vitamin A (retinol). Arch Dermatol 2007;143: 606-12.