Prevalence of extended spectrum beta-lactamase in Klebsiella pneumoniae

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Published: Sep 26, 2008
DOI: https://doi.org/10.18051/UnivMed.2008.v27.98-105 Universa Medicina
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Keywords:
Extended spectrum a-lactamase Klebsiella antibiotics

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Elly Herwana
Department of Pharmacology, Medical Faculty, Trisakti University
Yenny Yenny
Department of Pharmacology, Medical Faculty, Trisakti University
Laurentia Pudjiadi
Department of Pharmacology, Medical Faculty, Trisakti University
Juius E Surjawidjaja
Department of Microbiology Medical Faculty, Trisakti University
Murad Lesmana
Department of Microbiology Medical Faculty, Trisakti University

Abstract

Increased irrational use of antibiotics promotes bacterial resistance to these drugs. Among the resistance mechanisms developed by bacteria is the production of â-lactamase which can destroy the â-lactam ring and cause resistance to the other â-lactam antibiotics, such as amoxycillin. Extended spectrum â-lactamase (ESBL), an enzyme found in bacterial plasmids, is capable of hydrolyzing third-generation cephalosporins, namely cefotaxime, ceftazidime, ceftriaxone, and the monobactams. ESBL is predominantly found in Klebsiellas spp., Escherichia coli and other bacteria of the Enterobacteriaceae family. This study was conducted to determine the prevalence of Klebsiella resistant to cephalosporins and the prevalence of those producing ESBL. A total of 65 clinical isolates of Klebsiella were tested by the disk diffusion method according to Kirby-Bauer to determine their antibiotic susceptiblity and by the double-disk synergy method to detect the presence of ESBL. The results show that 18.5% of Klebsiella isolates tested were resistant to ceftazidime and cefixime, 13.9% to ceftriaxone, and 23.1% to aztreonam. Testing for ESBL revealed that the prevalence of ESBL producers in clinical Klebsiella isolates ranged from 10.8% to 12.3%. The presence of ESBL, plus the potential for plasmid-mediated quinolone and carbapenem resistance, undoubtedly will create significant therapeutic problems in the future.

Article Details

How to Cite
Herwana, E., Yenny, Y., Pudjiadi, L., Surjawidjaja, J. E., & Lesmana, M. (2008). Prevalence of extended spectrum beta-lactamase in Klebsiella pneumoniae. Universa Medicina, 27(3), 98–105. https://doi.org/10.18051/UnivMed.2008.v27.98-105
Issue
Vol. 27 No. 3 (2008)
Section
Review Article

The journal allows the authors to hold the copyright without restrictions and allow the authors to retain publishing rights without restrictions.

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