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Aloe vera extract reduces 8-oxo-2'-deoxyguanosine levels and improves total antioxidants in streptozotocin-induced diabetic rats

Wulan Christijanti, Aditya Marianti, Wiwi Isnaeni
Submission date: Friday, 09 December 2016
Published date: Friday, 07 April 2017
DOI: http://dx.doi.org/10.18051/UnivMed.2017.v36.34-41

Abstract


Background

Diabetes mellitus is a chronic disease caused by lack of insulin production in the pancreas or by insulin resistance, the disease being characterized by elevated blood glucose levels. Hyperglycemia in diabetes could lead to oxidative stress due to the rise in 8-oxo-2'-deoxyguanosine (8-oxo-dG) levels and the decrease in levels of total antioxidant status (TAS). The purpose of this study was to assess the effect Aloe vera extract on 8-oxo-dG level and total antioxidant status in diabetic rat testis.

 

Methods

This was an experimental laboratory study with 20 rat samples which were divided into 4 groups (1 control group and 3 treatment groups). Diabetes was induced in the rats by streptozotocin (STZ) at 65 mg/kgBW. The diabetic rats were then treated for 28 days with Aloe vera extract at 0 mg (P0), 200 mg rind (P1), 200 mg pulp (P2), respectively. The level of 8 -oxo-dG was measured by ELISA and total antioxidant status with 2,2' -azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS). Data were analyzed with ANOVA and Least Significant Difference Advanced Test at P<0.05.

 

Results

8-Oxo-dG levels were significantly different between the control group and both P0 and P2, but not between the control group and P1. Among the treatment groups the 8-oxo-dG levels were significantly different. Mean total antioxidant status was significantly different between control and treatment groups, and also between treatment groups (p<0.05).

 

Conclusions

Aloe vera extract reduced free radicals (level of 8-oxo-dG) and increased the total antioxidant status in diabetic rat testis.

 


Keywords


Aloe vera; 8-oxo-dG; total antioxidants; diabetes mellitus

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References


Giacco F, Brownlee M. Oxidative stress and diabetic complications. Circ Res 2010;107: 1058-70.

Turrens JF. Mitochondrial formation of reactive oxygen species. J Physiol 2003;552:335–44.

Sun J, Lou X, Wang H, et al. Serum 8-hydroxy-2′-deoxyguanosine (8-oxo-dG) levels are elevated in diabetes patients. Int J Diabetes Dev Ctries 2011;35:368-71.

Chan SS, Byung SM, Kyong SP, et al. Serum 8-hydroxy-guanine levels are increased in diabetic patients. Diabetes Care 2001;24:733–7.

Liu X, Gan W, Zou Y, et al. Elevated levels of urinary markers of oxidative DNA and RNA damage in type 2 diabetes with complications. Oxidative Med Cell Longevity 2016, Article ID 4323198,7 pages http://dx.doi.org/10.1155/2016/4323198.

Simone S, Gorin Y, Velagapudi C, et al. Mechanism of oxidative DNA damage in diabetes: tuberin inactivation and down regulation of DNA repair enzyme 8-oxo-7,8-dihydro-2-deoxyguanosine-DNA glycosylase. Diabetes 2008;57:2626–36.

Hussein J, El-Khayat Z, Taha M, et al. Insulin resistance and oxidative stress in diabetic rats treated with flaxseed oil. J Med Plants Res 2012;6:5499-06.

Smart DJ, Chipman JK, Hodges NJ. Activity of OGG1 variants in the repair of prooxidant-induced 8-oxo-2’-deoxyguanosine. DNA Repair 2006;5:1337–45.

Maritim A, Sanders R, Watkins J. Diabetes, oxidative stress, and antioxidants: a review. J Biochem Mol Tox 2003;17:24-38.

Pham-Huy LA, He H, Pham-Huy C. Free radicals, antioxidants in disease and health. Int J Biomed Sci 2008;4:89-96.

Wu JH, Ward NC, Indrawan AP, et al. Effects of α-tocopherol and mixed tocopherol supplementation on markers of oxidative stress and inflammation in type 2 diabetes. Clin Chem 2007;3:511-9.

Mandal S, Yadav S, Yadav S, et al. Antioxidants: a review. J Chem Pharm Res 2009;1:102-4.

Rajasekaran S, Sivagnanam K, Subramanian S. Antioxidant effect of Aloe vera gel extract in streptozotocin-induced diabetes in rats. Pharmacol Report 2005;57:90–6.

Rodriguez Rodriguez E, Darias Martin J, Diaz Romero C. Aloe vera as a functional ingredient in foods. Crit Rev Food Sci Nutr 2010;50:305-26.

Rahimifard M, Navaei-Nigjeh M, Mahroui N. Improvement in the function of isolated rat pancreatic islets through reduction of oxidative stress using traditional Iranian medicine. Cell J 2013;16:147-63.

Mohapatra S, Pradhan S, Rath B, et al. Antioxidant properties of Aloe vera in streptozotocin induced diabetic rats. Int J Pharm Bio Sci 2013;4:87–91.

Prueksrisakul T, Chantarangsu S, Thunyakitpisal P. Effect of daily drinking of Aloe vera gel extract on plasma total antioxidant capacity and oral pathogenic bacteria in healthy volunteer : a short-term study. J Complem Integr Med 2015;12:159–64. DOI: https://doi.org/10.1515/jcim-2014-0060.

World Health Organization. Definition and diagnosis of diabetes mellitus and intermediate hyperglycemia. Geneva, Switzerland: World Health Organization;2011.

Ghasemi A, Khalifi S, Jedi S. Streptozotocin-nicotinamide induced rat model of type 2 diabetes. Acta Physiol Hungaria 2014;101:408-20.

Enas AKM. Antidiabetic, antihypercholestermic and antioxidative effect of Aloe vera gel extract in alloxan induced diabetic rats. Aust J Basic Appl Sci 2011;5:1321-7.

Abo-Youssef AMH, Messiha BAS. Beneficial effects of Aloe vera in treatment of diabetes: comparative in vivo and in vitro studies. Bull Fac Pharm 2013;51:7–11.

Edogbanya IHN, Ocholi OJ, Apeji Y. Comparative study on the single and combined effects of leaf extracts of Aloe vera and Vernonia amygdalina on blood glucose level and histology of the pancreas in alloxan-induced diabetic Wistar rats. Adv Agriculture Sci Eng Res 2014;4:1687–94.

Roszkowski K, Jozwicki W, Blaszczyk P, et al. Oxidative damage DNA: 8-oxo Gua and 8-oxo dG as molecular markers of cancer. Med Sci Monit 2011;17:329- 33.

Kilarkaje N, Al-Hussaini H, Al-Bader MM. Diabetes-induced DNA damage and apoptosis are associated with poly (ADP ribose) polymerase 1 inhibition in the rat testis. Eur J Pharmacol 2014;737:29–40.

Araki E, Nishikawa T. Oxidative stress: a cause and therapeutic target of diabetic complications. J Diabetes Invest 2010;1:90-6.

Halliwell B. Free radicals and antioxidants – quo vadis? Trends Pharmacol Sci 2011;32:125- 30.

Halliwell B, Gutteridge JMC, editor. Free radicals in biology and medicine. 4th ed, Oxford: Clarendon Press;2007.

Kumar S. Free radicals and antioxidants: human and food system. Adv Appl Sci Res 2011;2:129- 35.

Ramachandraiahgari RMY, Somesula SR, Adi PJ, et al. Protective role of ethanolic extract of Aloe vera antioxidant properties on liver and kidney of streptozotocin-induced diabetic rats. Digest J Nanomaterials Biostructures 2012;7:175–84.

Darmawan H. Production of ROS and its effects on mitochondrial and nuclear DNA, human spermatozoa, and sperm function. Med J Indones 2007;16:127- 33.

Minamiyama Y, Takemura S, Bito Y, et al. Supplementation of α-tocopherol improves cardiovascular risk factors via the insulin signalling pathway and reduction of mitochondrial reactive oxygen species in type II diabetic rats. Free Radic Res 2008;42:261-71.


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