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Hepatoprotective effect of red ginger rhizome extract in deep frying oil-fed male wistar rats

Sunarti Sunarti, Edy Fachrial, Urip Harahap, Delyuzar Delyuzar, Tri Widyawati, Lokot Donna Lubis
Submission date: Thursday, 08 June 2017
Published date: Friday, 08 December 2017
DOI: http://dx.doi.org/10.18051/UnivMed.2017.v36.228-235

Abstract


BACKGROUND
Non-alcoholic steatohepatitis (NASH) is a chronic liver disease associated with increased free fatty acids, trans fatty acids and free radical compounds due to the consumption of deep frying oil (DFO). Ginger has hypolipidemic and antioxidant properties, and acts as an insulin sensitizer. The present study was conducted to evaluate the hepatoprotective effect of an ethanol extract of Zingiber officinale Roxb var rubrum (EEZO) in DFO-fed male Wistar rats.

METHODS
A study of experimental design was conducted involving 24 non-alcoholic steatohepatitis (NASH) male Wistar rats that were randomly divided into 4 groups. Group K1 (control) was given DFO-3 (3 times heated DFO) at a dose of 10 ìl/g BW/day. The treatment groups (P1, P2, P3) were given DFO-3 and red ginger rhizome extract at doses of 100, 200, and 400 mg/kg BW orally for 30 days. The histopathology examination assessed fatty degeneration, and degree of fibrosis. The data were analyzed with the Kruskal-Wallis and Mann-whitney test.

RESULTS    
The Kruskal-Wallis test showed that fatty degeneration (steatosis, lobular inflammation, hepatocyte ballooning and NASH) were significantly different among the four groups (p<0.05). Mann-Whitney test proved that fatty degeneration was not significantly different between control and treatment groups (p>0.05).

CONCLUSION
Deep frying oil increased fatty degeneration and NASH, and none of red ginger rhizome extract doses was able to inhibit fatty degeneration significantly. For the future research it is intended to prolong the induction time and the administration of red ginger rhizome extract.

Keywords


NASH; red ginger rhizome extract; fatty degeneration; fibrosis; rats

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References


Kelishadi R, Farajian S, Mirlohi M. Probiotics as a novel treatment for non-alcoholic fatty liver disease: a systematic review on the current evidences. Hepat Mon 2013;13:1–8.

Chalasani N, Younossi Z, Lavine JE, et al. The diagnosis and management of non-alcoholic fatty liver disease: practice guideline by the American Association for the Study of Liver Diseases, American College of Gastroenterology, and the American Gastroenterological Association. Hepatology 2012;55:2005–23.

Chowdhury A, Younossi ZM. Global epidemiology and risk factors for nonalcoholic fatty liver disease. In: Chalasani N, Szabo,editors. Alcoholic and non-alcoholic fatty liver disease. Geneva: Springer international Publishing Switzerland;2016.p. 21-404.

Farrell GC, Wong VW-S, Chitturi S. NAFLD in Asia-as common and important as in the West. Nat Rev Gastroenterol Hepatol 2013;10:307–18.

Oeij AA, Atmadja WL, Achmad S,et al. Gambaran anatomi mikroskopik dan kadar malondialdehida pada hati mencit setelah pemberian minyak kelapa sawit bekas menggoreng. JKM 2007;7:14–25.

Marinova EM, Seizova KA, Totseva IR, et al. Oxidative changes in some vegetable oils during heating at frying temperature. Bulg Chem Commun 2012;44:57–63.

Farag RS, Abdul-Latef MS, Basuny AMM, et al. Effect of non-fried and fried oils of varied fatty acid composition on rat organs. Agric Biol J N Am 2010;1:501-9.

Dewi R, Sutejo IR. Administration of used cooking oil to mice causes liver cell damage and decreases serum albumin. Moluca Medica 2011;4:61- 9.

Dhibi M, Brahmi F, Mnari A, et al. The intake of high fat diet with different trans fatty acid levels differentially induces oxidative stress and non alcoholic fatty liver disease (NAFLD) in rats. Nutr Metab 2011;8:65. doi: 10.1186/1743-7075-8-65.

Martin-Dominguez V, Gonzalez-Casas R, Mendoza-Jimenez-Ridruejo J, et al. Pathogenesis, diagnosis and treatment of non-alcoholic fatty liver disease. Rev Española Enfermedades Dig 2013;105:409–20.

Jurnalis YD, Sayoeti Y, Elfitrimelly. Role of antioxidants in non-alcoholic fatty liver disease (NAFLD). J Kesehatan Andalas 2014;3:15–20.

Shirin APR, Jamuna P. Chemical composition and antioxidant properties of ginger root (Zingiber officinale). J Med Plants Res 2010;4:2674–9.

Nammi S, Kim MS, Gavande NS, et al. Regulation of low-density lipoprotein receptor and 3-hydroxy-3-methylglutaryl coenzyme A reductase expression by Zingiber officinale in the liver of high-fat diet-fed rats. Basic Clin Pharmacol Toxicol 2010;106:389–95.

Haniadka R, Saxena A, Shivashankara AR, et al. Ginger protects the liver against the toxic effects of xenobiotic compounds: preclinical observations. J Nutr Food Sci 2013;3:226. doi: 10.4172/2155-9600.1000226.

El-Abhar HS, Hammad LN, Gawad HS. Modulating effect of ginger extract on rats with ulcerative colitis. J Ethnopharmacol 2008;118:367–72.

Setiawan A. Alpha lipoic acid oral inhibits improvement in number of steatosis and ALT levels in waste oil induced of male Wistar rat [tesis]. Denpasar: Universitas Udayana;2014.

Elrokh ESM, Yassin NAZ, El-Shenawy SMA, et al. Antihypercholesterolaemic effect of ginger rhizome (Zingiber officinale) in rats. Inflammopharmacology 2010;18:309–15.

Nwozo SO, Osunmadewa DA, Oyinloye BE. Anti-fatty liver effects of oils from Zingiber officinale and Curcuma longa on ethanol-induced fatty liver in rats. J Integr Med 2014;12:59–65.

Totani N, Ojiri Y. Mild ingestion of used frying oil damages hepatic and renal cells in Wistar rats. J Oleo Sci 2007;56:261-7

Departemen Kesehatan RI, Farmakope Indonesia. edition V. Jakarta: Departemen Kesehatan Republik Indonesia; 2014.

Yang Z, Yang W, Peng Q, et al. Volatile phytochemical composition of rhizome of ginger after extraction by headspace solid-phase microextraction, petrol ether extraction and steam distillation extraction. Bangladesh J Pharmacol 2009;4:136-43.

Wahyuningrum MR, Probosari E. The effect of papaya fruit (Carica papaya L.) on triglyceride levels in hypercholesterolemic Sprague Dawley rats. J Nutr Coll 2012;1:192-8 .

Novriansyah R. Differences in collagen density around wounds incision in conventional wrinkle and occlusive hydrocolloid closures for 2 and 14 days [thesis]. Semarang: Universitas Diponegoro; 2008.

Brunt EM. Nonalcoholic fatty liver disease: pros and cons of histologic systems of evaluation. Int J Mol Sci 2016;17: 97. doi: 10.3390/ijms17010097.

Hassan NA, Karunakaran R, Uma SA, et al. Anti-inflammatory effect of Zingiber officinale on Sprague Dawley rats. Asian J Pharm Clin Res 2017;10:3–5.

Malipatil NB, Manjunath S, Shruthi DP. Evaluation of effect of aqueous extract of Zingiber officinale Roscoe (Ginger) on acute and chronic inflammation in adult albino rats. Asian J Pharm Clin Res 2015;8:113–6.

Kumar S, Saxena K, Singh UN, et al. Anti-inflammatory action of ginger: A critical review in anemia of inflammation and its future aspects. Int J Herb Med 2013;116–20.

Stoilova I, Krastanov AI, Stoyanova A, et al. Antioxidant activity of a ginger extract (Zingiber officinale). Food Chem 2007;102:764–70.

Redha A. Flavonoids: structure, antioxidative properties and their roles in biological systems J Belian 2010;9:196-202.

Vamanu E. In vitro antimicrobial and antioxidant activities of ethanolic extract of lyophilized mycelium of Pleurotus ostreatus PQMZ91109. Molecules 2012;17:3653–71.

Adawiah, Sukandar D, Muawanah A. Antioxidant activity and content of bioactive components of namnam fruit juice. Valens J Chem 2015;1:127-33.

Sartika RAD. Effect of temperature and duration of frying (deep frying) on the formation of trans fatty acids. Makara Sains 2009;13:23-8.

Sartika RAD. Effects of saturated, unsaturated and trans fatty acids on health. Kes Mas 2008;2: 154-60.

Susianti. The effect of cooking oil refined with noni fruit (Morinda citrifolia) on the histopathology of rat liver and heart. MKA 2014; 37:54-60.

Bruhl L. Fatty acid alterations in oils and fats during heating and frying. Eur J Lipid Sci Technol 2014;116:707–15.

Labonte ME, Jenkins DJA, Lewis GF, et al. Adding MUFA to a dietary portfolio of cholesterol-lowering foods reduces apoAI fractional catabolic rate in subjects with dyslipidaemia. Br J Nutr 2013; 110:426–36.

Fouda A, Berika M. Evaluation of the effect of hydroalcoholic extract of Zingiber officinale rhizomes in rat collagen-induced arthritis. Basic Clin Pharmacol Toxicol 2009;104:262-71.


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