Phaleria macrocarpa reduces glomerular growth factor expression in alloxan-induced diabetic rats
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Abstract
Diabetic nephropathy (DN) is the most serious complication of diabetes, causing
end-stage renal disease throughout the world. Recent studies have reported a
direct role of vascular endothelial growth factor (VEGF) and transforming growth
factor-â (TGF-â) in DN pathogenesis. VEGF and TGF-â are expressed early in
glomeruli in response to hyperglycemia. Active substances of Phaleria
macrocarpa (PM) pericarp are known to have nephroprotective effects. This
study aimed to evaluate the effects of Phaleria macrocarpa (Scheff.) Boerl
pericarp extract on VEGF and TGF-â expression in alloxan-induced diabetic rats.
METHODS
An experimental study was conducted on twenty five male albino (Sprague
Dawley) rats divided into five groups (of five each): normal control; diabetic;
diabetic + metformin 100 mg/kgBW; diabetic + methanolic PM extract 250 mg/
kgBW; and diabetic + aqueous PM extract 250 mg/kgBW. Diabetes was induced
by alloxan monohydrate 150 mg/BW intraperitoneally. Treatment was given for
3 weeks. VEGF and TGF-â expression analysis was performed by means of
immunohistochemical technique. Differences between groups were assessed
by one-way ANOVA.
RESULTS
VEGF expression in the PM extract group was significantly lower than that in the
diabetic group and even metformin group (p<0.01). TGF-â expression in
methanolic PM extract group was significantly lower than in diabetic and
metformin group (p<0.01), but aqueous PM extract group only showed
significancy when compared with diabetic group (p< 0.01).
CONCLUSIONS
Phaleria macrocarpa pericarp extract reduces glomerular expression of TGF-â
and VEGF in alloxan-induced diabetic rats.
Article Details
The journal allows the authors to hold the copyright without restrictions and allow the authors to retain publishing rights without restrictions.
References
Wild S, Roglic G, Green A, Sicree R, King H. Global prevalence of diabetes. Estimates for the year 2000 and projections for 2030. Diabetes Care 2004;27:1047-53.
Ramachandran A, Snehalatha C, Samith SA, Nanditha A. Trends in prevalence of diabetes in Asian countries. World J Diabetes 2012;3:110-7.
Kanwar YS, Wada J, Sun L, Xie P, Wallner EI, Chen S, et al. Diabetic nephropathy: mechanism of renal disease progession. Exp Biol Med 2008;233:4-11.
Ohshiro Y, Lee Y, King GL. Mechanism of diabetic nephropathy: role of protein kinase C activation, Adv Stud Med 2005;2:S10-9.
Ghaisas MM, Navghare VV, Takawale AR, Zope VS, Phanse MA. Antidiabetic and nephroprotective effect of Tectona grandis Linn. in alloxan induced diabetes. Ars Pharm 2010;51:
-206.
Tervaert TWC, Mooyart AL, Mann K, Cohen AH, Cook HT, Crachenberg CB, et al. Pathologic classification of diabetic nephropathy. J Am Soc Nephrol 2010;1-8. doi: 10.1681/ASN.2010010010.
Di Loreto, Moreno HS, Puche RC, Locatto ME. Severe hyperglicemia: a determinant factor for hypofiltration in alloxan diabetic rats. Acta Diabetol 2004;41:56-63.
Brosius FC. Trophic factors and cytokines in early diabetic glomerulopathy. Exp Diab Res 2003;4:225-33.
Flyvbjerg A, Dagnaes-Hansen F, De Vriese AS, Schrijvers BF, Tilton RG, Rasch R. Amelioration of long-term renal changes in obese type 2 diabetic mice by a neutralizing vascular
endothelial growth factor antibody. Diabetes 2002;
:3090–4.
Ali R, Atangwho I, Kaur N, Abraika O, Ahmad M, Mahmud R, et al. Bioassay-guided antidiabetic study of Phaleria macrocarpa fruit extract. Molecules 2012;17:4986-5002.
Ali R, Item JA, Navneet K, Elsnoussi AHM, Ali JM, Mohd. ZA, et al. Hypoglycemic and antihyperglycemic study of Phaleria macrocarpa fruit pericarp. J Med Plant Res 2012;6:1982-90.
Sulistyoningrum E, Setiawati, Ismaulidiya FR. Phaleria macrocarpa (Scheff.) Boerl improved renal histological changes in alloxan-induced diabetic rats. Int J Med Plant Altern Med 2013;1:87-92.
Triastuti A, Hee-JP, Jong WC. Phaleria macrocarpa suppress nephropathy by increasing renal antioxidant enzyme activity in alloxaninduced diabetic rats. Nat Prod Sci 2009;15:167-72.
Sedigheh A, Jamal MS, Mahbubeh S, Somayeh K, Mahmoud RK, Azadeh A, et al. Hypoglycaemic and hypolipidemic effects of pumpkin (Cucurbita pepo L.) on alloxan-induced diabetic rats. Afr J Pharm Pharmacol 2011;5:2620-6.
Cha DR, Kang YS, Han SY, Jee YH, Han KH, Han JY, et al. Vascular endothelial growth factor is increased during early stage of diabetic nephropathy in type II diabetic rats. J Endocrinol
;183:183–94.
Gilbert RE, Cox A, Wu LL, Allen TJ, Hulthen UL, Jerums G, et al. Expression of transforming growth factor-â1 and type IV collagen in the renal tubulointerstitium in experimental diabetes.
Diabetes J 1998;47:414–22.
Langham RG, Kelly DJ, Gow RM, Zhang Y, Cordonnier DJ, Pinel N, et al. Transforming growth factor â in human diabetic nephropathy. Diabetes Care 2006;29:2670–5.
Teoh, SL, Latiff AA, Das S. Histological changes in the kidney of experimental diabetic rats fed with Momordica charantia (bitter gourd) extract. Rom J Morphol Embryol 2010;51:91-5.
Qian Y, Feldman E, Pennathur S, Kretzler M, Brosius FC. From fibrosis to sclerosis: mechanism of glomerulosclerosis in diabetic nephropathy. Diabetes J 2008;57:1439-45.
Hendra R, Ahmad S, Oskoueian E, Sukari A, Shukor MY. Antioxidant, anti-inflammatory and cytotoxicity of Phaleria macrocarpa (Boerl.) Scheff fruit. BMC Complement Altern Med 2011;17:4986-5002.
Elmarakby AA, Sullivan JC. Relationship between oxidative stress and inflammatory cytokines in diabetic nephropathy.Cardiovasc Ther 2010;30:1–11.
Krishan P, Chakkarwar VA. Diabetic nephropathy: aggressive involvement of oxidative stress. Pharm Educ Res 2011;2:35-41
Lee EY, Lee MY, Hong SW, Chung CH, Hong SY. Blockade of oxidative stress by vitamin C ameliorates albuminuria and renal sclerosis in experimental diabetic rats. Yonsei Med J 2007;48:
- 55.
Meiyanti HRD, Fransiscus DS. Efek hipoglikemik daging buah mahkota dewa (Phaleria macrocarpa (Scheff.) Boerl.) terhadap kadar gula darah pada manusia sehat setelah pembebanan
glukosa. Univ Med 2006;25:114-20.
Gonzalez-Gallego J, Sanchez-Campos S, Tunon MJ. Anti-inflammatory properties of dietary flavonoid. Nutr Hosp 2007;22:287-93.
Hendra R, Ahmad S, Sukari A, Shukor MY, Oskoueian E. Flavonoid analyses and antimicrobial activity of various parts of Phaleria macrocarpa (Scheff.) Boerl fruit. Int J Mol Sci
;12:3422-31.