Mucuna pruriens restores spermatogenesis in mice after exposure to 2-methoxyethanol

Main Article Content

Putu Oky Tania
Sri Winarni


Background Increasing industrial activity is causing many problems in reproductive health, such as infertility. The factors causing infertility are hormones, infection, radiation, drugs and chemicals. One of these is 2-methoxyethanol (2-ME). The objective of this study was to evaluate the effect of Mucuna pruriens (MP) seed fraction on spermatogenesis, including number of spermatogenic cells and spermatozoal membrane protein profiles after exposure to 2-ME in mice. Methods This study was of experimental design. Thirty mice were randomized into 5 groups, i.e. 2 control groups and 3 treatment groups. All mice were injected subcutaneously with 2-ME doses of 100mg/kg body weight (BW)/day, for a period of 12 days. Positive (PC) and negative controls (NC) were treated with carboxy methyl cellulose (CMC). The three treatment groups were given MP seed fraction in doses of 14 (T1), 28 (T2) and 56 mg/kg BW (T3), respectively, from day 13 until day 64. Data on spermatogenic cells were collected from histological cross-sections and analyzed with one-way ANOVA. The spermatozoal membrane protein profile was identified by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) with silver staining and were analyzed descriptively. Results Administration of MP seed fractions resulted in significantly different numbers of spermatocytes I and round spermatids between PC and T1, T2, and T3. A protein with the molecular weight of 41.6 kDa was expressed in all groups except the positive controls, while a 24.5 kDa protein was expressed in the positive controls only. Conclusion MP seed fraction effectively recovers spermatogenesis loss in mice due to 2-ME exposure.

Article Details

How to Cite
Tania, P. O., & Winarni, S. (2013). Mucuna pruriens restores spermatogenesis in mice after exposure to 2-methoxyethanol. Universa Medicina, 32(3), 137–145.
Review Article


Pasqualotto FF, Lucon AM, Sobreiro BP, Pasqualotto EB, Arap S. Effects of medical therapy, alcohol, smoking, and endocrine disruptors on male infertility. Rev Hosp Clin 2004;59:375-82.

Starek A, Miranowicz-Dzier¿awska K, Starek-Œwiechowicz1 B. Interactive effect of combined exposure to ethylene glycol ethers and ethanol on hematological parameters in rats. Health 2010;2:1054-64.

Irnidayanti Y. Expression of vimentin protein and neurofilaments on forelimb buds of black-6 mice on gestation day 12 induced by 2-Methoxyethanol by RT-PCR. Biosci 2010;2:116-20.

Supadmi. Efek 2-methoxyethanol terhadap kadar testosteron dan histologis testis mencit (Mus musculus L) [tesis]. Surabaya: Universitas Airlangga; 2009.

Tirado OM, Martinez ED, Rodriguez OC, Danielsen M, Selva DM, Reventos J, et al. Methoxyacetic acid disregulation of androgen receptor and androgen-binding protein expression in adult rat testis. Biol Reprod 2003; 68:1437-46.

William HW, Cheng J. FSH and testosterone signaling in sertoli cells. Reprod 2005;130:15–28.

Mikkila TFM, Toppari J, Paranko J. Effects of neonatal exposure to 4-tert-octylphenol diethylstilbestrol, and flutamide on steroidogenesis in infantile rat testis. Toxicol Sci 2006;91:456–66.

Hayati A, Mahmuda A, Ray Pidada IB. 2-Methoxyethanol exposure affects protein profile of testicular sperm membrane in mice (Mus musculus). Folia Medica Indonesiana 2010;46: 253-26.

Sharma BK, Ahmad S, Singh R, Verma RK, Kumar N. A review on Mucuna pruriens: its phyto-constituents and therapeutic uses. Int J Pharm Sci 2012;1:308–12.

Shukla KK, Mahdi AA, Ahmad MK, Jaiswar SP, Shankwar SN, Tiwari SC. Mucuna pruriens reduces stress and improves the quality of semen in infertile men. Evid Based Complement Alternat Med 2010;7:137–44.

Singh AP, Sarkar S, Tripathi M, Rajender S. Mucuna pruriens and its major constituent L-DOPA recover spermatogenic loss by combating ROS, loss of mitochondrial membrane potential and apoptosis. Plos One 2013;8:e54655. doi: 10. 1371/journal.pone.0054655.

Pugalenthi M, Vadivel V, Siddhuraju P. Alternative food/feed perspectives of an underutilized legume Mucuna pruriens var. utilis: a review. Plant Foods Hum Nutr 2005;60: 201-18.

Speroff L, Fritz MA. Clinical gynecology endocrinology and infertility. 7th ed. Philadelphia: Lippincot Williams & Wilkins; 2005.

Sudiana IK. Teknologi ilmu jaringan dan imunohistokimia. Jakarta: Penerbit Sagung Seto; 2005.

Hess AR, Renato de Franca L. Spermatogenesis and cycle of the seminiferous epithelium. Adv Exp Med Biol 2008;636:1-15. doi: 10.1007/978-0-387-09597-4_1.

Hayati A, Any D, Rai Pidada IB. Spermatozoa motility and morphological recovery process in mice after the induction of 2-methoxyethanol. Folia Medica Indonesia 2005;41:90-5.

Barone F, Aguanno S, Agostino D. Modulation of MAA-induced apoptosis in male germ cells: role of Sertoli cell P/Q-type calcium channels. Reprod Biol Endocrinol 2005;3:13-23.

Bagchi G, Waxman DJ. Toxicity of ethylene glycol monomethyl ether: impact on testicular gene expression. Int J Androl 2008;31:269–74.

Office of Environmental Health Hazard Assessment (OEHHA). Proposition 65 maximum allowable dose level (MADL) for reproductive toxicity for ethylene glycol monomethyl ether; 2004.

Honda A, Yamagata K, Sugiura S, Watanabe K, Baba T. A mouse serine protease TESP5 is selectively included into lipid rafts of sperm membrane presumably as a glycosylphosphatidy linositol-anchored protein. Biol Chem 2002;227: 16976-84.