Red fruit oil supplementation fails to prevent oxidative stress in rats
Article Sidebar
Main Article Content
Abstract
Red fruit oil (RFO) is popular in Indonesia especially in Papua. It is consumed by people for maintaining health and to treat diseases. Red fruit (Pandanus conoideus Lam) oil is reported to contain the antioxidants á-tocoferol and âcarotene.
The aim of this study was to confirm the protective effect of RFO
against decreased glutathione levels in the plasma and liver of rats induced by 2N-(2-fluorenyl) acetamide (2-FAA).
METHODS
Wistar male rats weighing 200 ± 20 g were randomly assigned into a control group (receiving distilled water only) and three intervention groups, designated RFO, FAA, and RFO-FAA (n=6 rats per group). RFO was given RFO 10 ì l/g/
BW/day, FAA received fluoroenylacetamide (FAA) at 40ìg/day, while RFOFAA received both RFO and FAA. At 4 weeks blood samples were taken from the tail. At 8 weeks the rats were sacrified for collection of blood and liver tissues. Ellman’s method was employed to determine the parameters of antioxidant
glutathione (glutahione sulfhydryl/GSH). One-way ANOVA and Tukey post hoc test were used to compare glutathione levels between groups.
RESULTS
This study showed that liver and plasma glutathione levels were not significantly lower in the RFO-FAA group than in the FAA group. Glutathione levels were significantly lower in plasma and liver homogenates of the RFO group compared with the control group and were not significantly different from those in the
FAA group.
CONCLUSION
Administration of RFO in rats does not protect against decreased glutathione but is a potential source of oxidative stress.
Article Details
The journal allows the authors to hold the copyright without restrictions and allow the authors to retain publishing rights without restrictions.
References
Hazra B, Biswas S, Mandal N. Antioxidant and free radical scavenging activity of Spondia pinnata. BMC CAM 2008;8:63. doi:10-1186/1472-6882-8063.
Kunwar A, Priyadarsini KI. Free radical, oxidative stress and importance of antioxidants in human health. J Med Allied Sci 2011;1:53-60.
Berger MM. Can oxidative damage be treated nutritionally? Clin Nutr 2005;24:172-84.
Moeloek FA. Herbal and traditional medicine: national perspectives and policies in Indonesia. J Bahan Alam Indonesia 2008;5:293-7.
Limbongan J, Malik A. Opportunity of red fruit crop (Pandanus conoideus Lamk.) development in Papua Province. J Penelitian Pengembangan Pertanian 2009;28;134-41.
Arumsari NI, Riyanto S, Rohman A. Some physico-chemical properties of red fruit oil (Pandanus conoideus Lam) from hexane and chloroform fractions. J Food Pharm Sci 2013;1:30-
Rohman A, Sugeng R, Che Man YB. Characterization of red fruit (Pandanus conoideus Lam) oil. Int Food Res J 2012;19:563-7.
Rohman A, Riyanto S, Yuniarti N, Saputra WR, Utami R, Mularsih W. Antioxidant activity, total phenolic and total flavonoid of extracts and fractions of red fruit (Pandanus conoideus Lam).
Int Food Res J 2010;17:97-106.
Syarkiah, Fitri LE, Pudjirahayu A. The effect of red fruit (Pandanus conoideus Lam) oil toward the formation of foam cells in aorta of Wistar strain rat (Rattus norvegicus) with atherogenic diet. J Kedokter Brawijaya 2008;23:6.
Sukmarini L. Konsentrasi glutation tereduksi (GSH) plasma tikus putih (Rattus norvegicus L.) jantan galur Wistar yang mengalami karsinogenesis sebagai akibat induksi N-2-
fluorenilasetamida. [skripsi]. Jakarta: Universitas Indonesia;2004.
Mei ZH, Hua RH, Tao LH. Progress in the research of GSH in cells. Chi Sci Bulletin 2011;56:3057-63.
Townsend DM, Tew KD, Tapiero H. The importance of glutathione in human disease. Biomed Pharmacother 2003;57:145-55.
Mun’im A, Andrajati R, Susilowati H. Tumorigenesis inhibition of water extract of red fruit (Pandanus conoideus Lam) on Sprague-Dawley rat female induced by 7,12 dimetilbenz(a)
antrasen (DMBA). J Pharm Sci 2006;3:153-61.
Yulhaeka S. Perbandingan daya antioksidan beberapa minyak buah merah (Pandanus conoideus Lam.) pada eritrosit domba yang mengalami stres oksidasi secara in vitro. [skripsi].
Jakarta: Universitas Indonesia;2006.
Liu P, Guo WS, Pu HC, Feng CN Zhu XK, Peng YX. Effects of high temperature on antioxidant enzymes and lipid peroxidation in flag leaves of wheat during grain filling period. Agric Sci China
;5:425-30.
James LP, Mayeux PR, Hinson JA. Acetaminophen-induced hepatotoxicity. Drug Metabol Dispos 2003;31:1499-506.
Thomson RW, Valentine HL, Valentine WM. In vivo and in vitro hepatotoxicity and glutathione interactions of N-methyldithiocarmbate and N, N-dimethyldithio-carbamate in the rat. Toxicol Sci 2003;70:269-80.
Mukhyarjon, Septelia, Parwati, Nurjati. Antikarsinogenesitas vs hepatotoksisitas minyak buah merah (MBM) pada tikus yang diinduksi fluoroenilasetamida (FAA). J Ilmiah Kedokter
; 1:1-8.
Djuartina T. Pengaruh pemberian minyak buah merah (Pandanus conoideus Lam.) pada hati tikus yang cedera akibat D-galaktosamin. [thesis]. Jakarta: Universitas Indonesia;2006.
Thomas C, MackeyMM, Diaz AA, Cox DP. Hydroxyl radical is produced via the Fenton reaction in submitochondrial particles under oxidative stress: implications for diseases associated with iron accumulation. Redox Rep 2009;14:102-8.
Oliveira BF, Veloso CA, Machado JA, Chaves M. High doses of in vitro beta-carotene, alphatocopherol and ascorbic acid induce oxidative stress and secretion of IL-6 in peripheral blood
mononuclear cells from healthy donors. Curr Aging Sci 2012;5:148-56.
Bjelakovic G, Nikolova D, Gluud LL, Simonetti RG, Gluud C. Mortality in randomized trials of antioxidant supplements for primary and secondary prevention. JAMA 2007;297:842-57.
He Y, Wang K, Wang L. Effect of tocopherol and carotene supplementation on meat quality and antioxidant capacity of pigs fed high-linseed oil diet. J Anim Plant Sci 2010;20:180-8.
Mathew M, Tay E, Cusi K. Elevated plasma free fatty acids increase cardiovascular risk by inducing plasma biomarkers of endothelial activation, myeloperoxidase and PAI-1 in healthy
subjects. Cardiovasc Diabetol 2010;9:1-9.
Tripathy D, Mohanty P, Dhindsa S, Syed T, Ghanim H, Alda A. Elevation of free fatty acids induces inflammation and impairs vascular reactivity in healthy subjects. Diabetes 2003;52:
-7.