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Nicotine supplementation blocks oocyte maturation in Rattus norvegicus

Meitria Syahadatina Noor, H.M Bahkriansyah, Widjiati Widjiati, Budi Santoso
Submission date: Wednesday, 02 December 2015
Published date: Wednesday, 14 August 2013
DOI: http://dx.doi.org/10.18051/UnivMed.2013.v32.92-98

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Abstract


BACKGROUND
Indonesia has the third largest tobacco consumption in the world after China and India. Nicotine as the main component of cigarette smoke has negative effects on the reproductive system, such as oocyte maturation, ovulation, and fertilization, and increasing the diploidy of oocytes. The goal of this research
was to evaluate the effect of nicotine on oocyte maturation in Rattus norvegicus.
METHODS
This was an experimental study with post test only control group design. The subjects were 40 rats selected homogenously and randomly. They were divided into a control group (receiving carboxy-methyl-cellulose sodium and 3 treatment groups (I-III) receiving nicotine subcutaneously for 7 days at dosages of 21 mg/ kgBW, 41 kg/kgBW and 84/kgBW, respectively. The observations comprised oocyte maturation stage, viz. germinal vesicle (GV), germinal vesicle breakdown (GVBD), metaphase I and metaphase II. Data were analyzed by one-way Anova with á=0.05, followed by Tukey’s HSD test.
RESULTS
One-way Anova showed significant differences in oocyte  maturation in all groups. Tukey’s HSD test showed that for GV, the differing groups were control and I, control and II, I and III. For GVBD, the differing groups were control and I, I and
II, I and III. For metaphase I, the differing groups were control with I, II, and III, I and II, I and III. For metaphase II, the differing groups were control versus I, II, and III, I and II, I and III.
CONCLUSION
Low dose of nicotine is capable of affecting oocyte maturation in Rattus norvegicus.

Keywords


Nicotine; maturation; oocyte; Rattus norvegicus

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References


Rabinoff M, Caskey N, Rissling A, Park C. Pharmacological and chemical effects of cigarette additives. Am J Public Health 2007;97:1981-8.

Hukkanen J, Jacob P III, Benowitz NL. Metabolism and disposition kinetics of nicotine. Pharmacol Rev 2005;57:79-115.

Vasquez J. Human rights and health: persons exposed to second-hand tobacco smoke. Washington DC: Pan American Health Organization; 2008.

Bordel R, Laschke MW, Menger MD, Vollmar B. Nicotine does not affect vascularization but inhibits growth of freely transplanted ovarian follicles by inducing granulosa cell apoptosis.

Hum Reprod 2006;21:610-6.

Talbot P, Riveles K. Smoking and reproduction: the oviduct as a target of cigarette smoke. Reprod Biol Endocrinol 2005;3:52. doi:10.1186/1477-7827-3-52.

Bordel R, Laschke MW, Menger MD, Vollmar B. Nicotine does not affect vascularization but inhibits growth of freely transplanted ovarian follicles by inducing granulosa cell apoptosis.

Hum Reprod 2006;21:610-7.

Mailhes JB, Young D, Caldito G, London SN. Sensitivity of mouse oocytes to nicotine induced perturbations during oocyte meiotic maturation and aneuploidy in vivo and in vitro. Mol Hum

Reprod 2000;6:232-7.

Dwirahayu Y. Pengaruh nikotin terhadap tingkat maturasi oosit pada tikus (Rattus novergicus) [thesis]. Surabaya: Universitas Airlangga; 2010.

Kakisina P. Pengaruh nikotin terhadap perkembangan embrio mencit (Mus musculus) [tesis]. Surabaya: Universitas Airlangga;2003.

Kusumawati D. Bersahabat dengan hewan coba. Yogyakarta: Gadjah Mada University Press; 2004.

Shah H. How to calculate the sample size for animal

studies.? Nat J Physiol Pharm Pharmacol 2011;1:35-9.

Mokhtar N, Rajikin M, Zakaria Z. Role of tocotrienol-rich palm vitamin E on pregnancy and preimplantation embryos in nicotine-treated rats. Biomed Res 2009;19:181-4.

Jennings PC, Merriman JA, Beckett EL, Hansbro PM, Jones KT. Increased zona pellucida thickness and meiotic spindle disruption in oocytes from cigarette smoking mice. Hum Reprod

;26:878-84.

Zenzes MT, Bielecki R. Nicotine-induced disturbances of meiotic maturation in cultured mouse oocytes: alterations of spindle integrity and chromosome alignment. Tob Induced Dis

;2:151-61.

Asadi E, Jahanshahi M, Golalipour MJ. Effect of vitamin E on oocytes apoptosis in nicotine-treated mice. Iran J Basic Med Sci 2012;15:880-7.

Son WY, Lim JH. Fertilization, cleavage and blastocyst development according to the maturation timing of oocytes in invitro maturation cycles. Hum Reprod 2005;20:3204-7.

Mrazek M, Fulka Jr J. Failure of oocyte maturation:

possible mechanisms for oocyte maturation arrest. Hum Reprod 2003;18:2249-52.


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Copyright (c) 2015 Meitria Syahadatina Noor, H.M Bahkriansyah, Widjiati Widjiati, Budi Santoso

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Universa Medicina by Faculty of Medicine, Trisakti University is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Based on a work at https://univmed.org/ejurnal/index.php/medicina/


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