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Apoptosis plays an important role in tumorigenesis. Induction of apoptosis is a strategy for developing cancer therapy. In vitro study found that andrographolide isolated from Andrographis paniculata has anticancer activity by an apoptotic mechanism in cancer cell lines. The aim of the present study was to prove the
effect of Andrographis paniculata extract administered orally on apoptosis of mammary adenocarcinoma in C3H mice.
This study was of post test randomized control group design. Twenty four C3H mice with transplanted mammary adenocarcinomas were divided into four groups. To three groups Andrographis paniculata extract was administered orally for
14 days, at doses of 5, 10 and 15 mg/day, respectively, whereas to the control group no Andrographis paniculata extract was administered. On day 15 the mice were terminated. The mammary adenocarcinomas were examined by the terminal deoxynucleotide transferase dUTP nick end labeling (TUNEL) method. The values of the apoptotic index were expressed as mean±SD and analyzed using ANOVA and Pearson’s correlation test.
The mean apoptotic index values differed significantly among the experimental groups (p=0.001). The highest value was found in the group receiving Andrographis paniculata extract 15 mg/day, while the lowest was in the control group, the values being significantly correlated (r=0.974).
Oral administration of Andrographis paniculata extract induced apoptosis in C3H mice with mammary adenocarcinoma
Williamson KE, El Din OS, O’Kane HF. Apoptosis. In: The Cancer Handbook. 2nd ed. Alison MR, editor. New York: John Wiley & Sons Ltd;2007.p.51-63.
Ferreira CG, Epping M, Kruyt FAE, Giaccone G. Apoptosis: target of cancer therapy. Clin Cancer Res 2002;8:2024-34.
World Health Organization. Guidelines for Management of Breast Cancer. EMRO Technical Publication Series; 2006.
World Health Organization. Estimated. agestandardised
incidence and mortality rate. Available at: http://www.globocan.iarc.fr/. Accessed June 25, 2013.
Departemen Kesehatan RI.. Profil kesehatan Indonesia tahun 2006. Jakarta: Departemen Kesehatan R.I.;2007.
Pandey MK, Singh GN, Sharma RK, Lata S. Physicochemical standardization of Andrographis paniculata (Nees): an Ayurvedic
drug. IJPRD 2011;3:81-9.
Saranya P, Geetha A, Selvamathy N. A biochemical study on the gastroprotective effect of andrographolide in rats induced with gastric ulcer. Res Paper 2011;73:550-7.
Sivaraj A, Vinothkumar P, Sathinyraj K, Sundarsen
S, Devi K, Senthilkumar B. Hepatoprotective potential of Andrographis paniculata aqueous leaf extract on ethanol induced liver toxicity in albino rats. JAPS 2011;1:204-8.
Ventriselvan S, Subasini U, Rajamanikam VC, Thirumuruja S. Hepatoprotective activity Andrographis paniculata in ethanol induced hepatotoxicity in albino Wistar rats. IJCP 2011;1:1-4.
Suebsana S, Pongnaratorn P, Sattayasai J, Arkaravichien T, Tiamkao S, Aromdee C. Analgesic, antipyretic, anti-inflammatory, and toxic effect of andrographolide derivates in experimental animals. Arch Pharmacol Res 2009;
Chao WW, Lin BF. Isolation and identification of bioactive compounds in Andrographis paniculata (Chuanxinlian). CMJ 2010;5:17. doi: 10.1186/1749-8546-5-17.
Sukardiman, Rahman A, Ekasari W, Sismindari. Induksi apoptosis senyawa andrographolida dari sambiloto (Andrographis paniculata Nees) terhadap kultur sel kanker. Media Kedokteran Hewan 2005;21:105-10.
Sukardiman, Harjotaruno, Widyawariyanti A, Sismindari, Zaini NC. Apoptosis inducing effect of andrographolide on TD-47 human breast cancer cell line. Afr J Tradit CAM 2007;4:345-51.
Nugrahaningsih, Tjahjono, Dharmana E. Apoptosis sel adenokarsinoma mamma mencit C3H setelah pemberian ekstrak sambiloto (Andrographis paniculata) (penelitian in vitro).
Media Medika Indonesiana 2003;38:121-4.
Martins CF, Dode MN, Báo SN, Rumpf R. The use of the acridine orange test and the TUNEL assay to assess the integrity of freeze-dried bovine spermatozoa DNA. Genet Mol Res 2007;6:
Shojaei F, Ferrara N. Role of the microenvironment in tumor growth and in rerfactoriness/resistance to anti-angiogenic therapies. Drug Resist Updat 2008;11:219-30.
Natal C, Osés-Prieto JA, Pelacko B, Iraburu MJ, Lopez-Zabaiza MJ. Regulation of apoptosis by peptides of fibronectin in human monocytes. Apoptosis 2006;11:209-19.
Sheeja K, Guruvayoorappan C, Kuttan G.. Antiangiogenic activity of Andrographis paniculata extract and andrographolide. Int Immunopharmacol 2007;7:211-21.
Al-Dissi AN, Haines DM, Singh B, Kidney BA. Immunohistochemical expression of vascular endothelial growth factor and vascular endothelial growth factor receptor-2 in canine
simple mammay gland adenocarcinoma. Can Vet J 2010;51:1109-14.
Vaculova A, Zhivotovsky B. Caspases: determination of their activities in apopotic cells. Methods Enzymol 2008;442:157-81.
Hakim L. Farmakokinetik klinik.Yogyakarta: Bursa
Shargel L, Wu-Pong S, Yu ABC. Applied biopharmaceutics and pharmacokinetics. 5th ed. Boston:McGraw-Hill Medical Publishing Division;2005.
Naidu SR, Ameer OZ, Salman IW, Venkatesh G, Sadikun A, Asmawi MZ. Pharmacokinetic study of androhraphis paniculata chloroform extract in rats. Pharmacol 2009;1:309-19.
Jarukamjorn K, Nemoto N. Pharmacological aspect of Andrographis paniculata on health and its major diterpenoid constituent andrographolide. Health Sci 2008;54: 370-81.
Zhou J, Lu GD, Ong CS, Ong CN, Shen HM. Andrographolide sensitizes cancer cells to TRAIL–induced apoptosis via p53-mediated death receptor 4 up-regulation. Mol Cancer Ther
Wong HC, Sagineedu SR, Lajis NH, Loke SC, Stanslas J. Andrographolide induces cell cycle arrest and apoptosis in PC-3 prostate cancer cell. Afr J Pharm Pharmacol 2011;5:225-33