Production of tumor necrosis factor-a is increased in urinary tract infections

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Published: 2012-12-14
DOI: https://doi.org/10.18051/UnivMed.2012.v31.167-174
Keywords:
Urinary tract infection, E. coli, serum TNF-á, TNF-á gene polymorphism

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Neni Susilaningsih
Department of Histology, Faculty of Medicine, Diponegoro University
Bambang Joni Karjono
Department of Internal Medicine, Dr. Kariadi Central Hospital/ Faculty of Medicine, Diponegoro University
Ratna Damma Purnamawati
Department of Histology, Faculty of Medicine, Diponegoro University

Abstract

BACKGROUND Urinary tract infection (UTI) is a common source of bacteriemia. The most common cause of UTI is Escherichia coli (E. coli). Tumor Necrosis Factor (TNF)-á gene polymorphism has been reported to be responsible for an excessive production of TNF-á and eventual disruption of pro-inflammatory cytokine regulation. The aim of this study was to compare TNF-á serum levels and TNF-á allele polymorphisms in patients with UTI due to E.coli and in non- UTI controls. METHODS A cross-sectional study was conducted at Dr. Kariadi Central Hospital and the Center for Biomedical Research, Faculty of Medicine, Diponegoro University, Semarang. In 68 patients with UTI the TNF-á serum levels were determined by means of ELISA and compared to those of non-UTI controls (n=55). TNFá- 308G>A gene polymorphism was analyzed by polymerase chain reaction restriction fragment length using the NcoI enzyme. Fragments were visualized on polyacrylamide gel with silver staining. RESULTS TNF-á serum level in patients with UTI had a median of 8.9 pg/mL, which was significantly higher than the median of 3.7 pg/mL in the control group (pA gene polymorphisms found in the patient group were G/G=61 (90%), G/A=7(10%) and A/A=0, while in the control group were G/G=48 (87%), G/A=7 (13%) and A/A =0. There was no significant differences (p=0.578) in gene polymorphisms between the two groups. CONCLUSIONS TNF-á serum levels in patients with UTI due to E. coli were significantly higher than in non-UTI controls, but for the TNF-á-380 gene polymorphisms no significant difference was found between the two groups. There are presumably more important factors than host genotype that influence UTI pathogenesis.

Article Details

Issue

Vol. 31 No. 3 (2012)

Section

Review Article

The journal allows the authors to hold the copyright without restrictions and allow the authors to retain publishing rights without restrictions.

How to Cite

Production of tumor necrosis factor-a is increased in urinary tract infections. (2012). Universa Medicina, 31(3), 167-174. https://doi.org/10.18051/UnivMed.2012.v31.167-174

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