Scurrula atropurpurea increases nitric oxide and decreases malondialdehyde in hypertensive rats

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Nour Athiroh AS
Erna Sulistyowati


Hypertension is the most prevalent chronic disease and has an impact on one billion people. Production of superoxide radicals and endothelial dysfunction are involved in hypertension. Scurrula atropurpurea (BL.) Dans. is a tea plant parasite. This study aimed to evaluate the role of Scurrula atropurpurea (BL.)
Dans. on nitric oxide (NO) as a marker of endothelial dysfunction and malondialdehyde (MDA) as a marker of oxidative stress in hypertensive rats.
This study subjected rats to deoxycorticosterone acetate (DOCA)-induced hypertension. The experimental groups consisting of the control group and 3 hypertension groups receiving Scurulla  tropurpurea extract at a dosage of 50; 100; and 200 mg/KgBW. Scavenging activity of Scurrula atropurpurea (BL.) extract was analyzed by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. The levels of arterial nitric oxide (NO) and pulmonary  malondialdehyde (MDA) were analyzed by spectrophotometry. ANOVA and a post hoc test were applied to find the difference of arterial NO and pulmonary MDA levels between groups.
The level of arterial NO was significantly decreased in the hypertension groups as compared with the control group, while the level of pulmonary MDA was significantly increased (p<0.05). Scurulla atropurpurea significantly increases the NO level at a dosage of 200 mg/KgBW, compared with the hypertension groups (p<0.001). Scurulla atropurpurea significantly decreases pulmonary MDA level at a dosage of 100 and 200 mg/KgBW compared with the hypertension groups (p<0.05).
Scurulla atropurpurea extract increases arterial NO and decreases pulmonary MDA in hypertensive rats, thus playing an important role in endothelial dysfunction and oxidative stress.

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How to Cite
AS, N. A., & Sulistyowati, E. (2013). Scurrula atropurpurea increases nitric oxide and decreases malondialdehyde in hypertensive rats. Universa Medicina, 32(1), 44–50. - 50
Review Article


Zhou W, Longhurst JC. Neuroendocrine mechanisms of acupuncture in the treatment of hypertension. Evid Based Complement Altern Med 2012;9 pages. doi:10.1155/2012/878673

Iwalokum BA, Hodonu SA, Nwoke S, Ojo O, Agomo PU. Evaluation of the possible mechanisms of antihypertensive activity of Loranthus micranthus: an African mistletoe. Biochem Res Int 2011;9 pages. doi:10.1155/2011/159439.

Szasz T, Lindr AE, Davis RP, Burnett R, Fink GD, Watts SW. Allopurinol does not decrease blood pressure or prevent the development of hypertension in the development of hypertension

in the DOCA-salt rat model. J Cardiovasc Pharmacol 2010;56:627-34.

Schulz E, Jansen T, Wenzel P, Daiber A, Münzel T. Nitric oxide, tetrahydrobiopterin, oxidative stress, and endothelial dysfunction in hypertension. Antioxid Redox Signal 2008;10:1115–26.

Szasz T, Watts SW. Uric acid does not affect the acetylccholine-induced relaxation of aorta from mormotensive and deoxycorticosterone acetatesalt hypertensive rats. J Pharmacol Exp 2010;333:758-63.

Ohashi K, Winarno H, Mukai M. Inoue M, Prana MS, Simanjuntak P, Shibuya H. Cancer cell invasion inhibitory effects of chemical constituetnts in the parasitic plant Scurrula

atropurpurea (Loranthaceae). Chem Pharm Bull 2003;51:343-5.

Badyal H, Lata H, Dadhich AP. Animal models of hypertension and effect of drugs. Indian J Pharmacol 2003;35:349-62.

Fard SG, Shamsabadi FT, Emadi M, Meng GY, Muhammad K, Mohamed S. Ethanolic extract of Eucheuma cottonii promotes in vivo hair growth and wound healing. J Anim Vet Sci 2011;10:601-5.

Duan XJ, Zhang WW, Li XM, Wang BG. Evaluation of antioxidant property of extract and fractions obtained from a red alga, Polysiphonia urceolata. Food Chem 2006;95:37–43.

Rosenblat M, Volkova N, Aviram M. Pomegranate phytosterol (β-sitosterol) and polyphenolic antioxidant (punicalagin) addition to statin significantly protected against macrophage foam cell formation. Atherosclerosis 2013; 226:110-17.

Murphy KJ, Chronopolous AK, Singh I, Francis MA, Moriarty H, Pike MI, et al. Dietary flavanols and procyanidin oligomers from cocoa (Theobroma cacao) inhibit platelet function. Am

J Clin Nutr 2003;6:1466-73.

Fenning A, Harrison G, Rose’meyer R, Hoey A, Brown L. l-arginine attenuates cardiovascular impairment in DOCA-salt hypertensive rats. Am J Physiol Heart Circ Physiol 2005;289:1408-16.

Schmitt VM, Dirsch VM. Modulation of endothelial nitric oxide by plant-derived product. Nitric Oxide 2009;21:77-91.

Steffen YM, Schewe T, Sies H. (-)-Epicatechin elevates nitric oxide in endothelial cells via inhibitins of NADPH oxidase. Biochem Biophys Res Comm 2007;359:828-33.

Kiel EC, Benkirane K, Javeshghani D, Touyz RM, Schiffrin EL. Xanthine oxidase and mitochondria contribute to vascular superoxide anion generation in DOCA-salt hypertensive rats. Am J Physiol Heart Circ Physiol 2008;295:281-8.

Hong CO, Lee HA, Rhee CH, Choung SY, Lee KW. Separation of the antioxidant compound quercitrin from Lindera obtusiloba Blume and its antimelanogenic effect on B16F10 melanoma

cells. Biosci Biotechnol Biochem 2013;1:58-64.

Piao MJ, Yoo ES, Koh YS, Kang HK, Kim J, Kim YJ, et al. Antioxidant effects of the ethanol extract from flower of Camellia japonica via scavenging of reactive oxygen species and induction of antioxidant enzymes. Int J Mol Sci 2011;12:2618 - 30.

Nakayama M, Aihara M, Chen YN, Araie M, Tomita-Yokotani K, Iwashina T. Neuroprotective effects of flavonoid on hypoxia-,glutamate-, and oxidative stress-induced retinal ganglion cell death. Mol Vis 2011;17:1784-93.

Stangl V, Dreger H, Stangl K. Moleculer target of tea polyphenols in the cardiovascular system. Cardiovasc Res 2007;739:348-58.