Aminolevulinate dehydrogenase polymorphisms did not modified lead serum and memory relationship

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Lantip Rujito
Arini Dewi Setyowati
Saien Saien


BACKGROUND Lead accumulation in the blood widely known affecting the formation of heme and oxygen transport processes in vital organs, Leading to organ failure including the brain synapses. Lead affinity has been recognized influenced by constitutional genotype of aminolevulinate dehydrogenase (ALAD), which encodes for heme synthesis. This research aimed to determine the relationship between plumbum (Pb) and short term memory on each ALAD gene genotyping (ALAD 1-1, ALAD 1-2 or ALAD 2-2) in gas station workers. METHODS Seventy six probands from gas station workers were recruited to participate in this research. Each probands was carried out ALAD genotyping using polymerase chain reaction-restriction fragment length polymorphism (PCRRFLP) method, lead serum level using atomic absorbent spectrophotometer (AAS), and short term memory was measurement by intelligence structure test (IST). RESULTS Proportion of δ ALAD 1-1, 1-2, and 2-2 were 91.8%, 8.2% and 0% respectively. Lead serum showed 15.84 ppb in homozygous 1-1, and 20.79 ppb in heterozygous. Short term memory in the probands varied from 85 until 117, with average in 99.71. There was significant negative relationship between lead serum and short term memory (r=-0.24; p=0.038). However, we could not find any significant correlation in each δ ALAD genotypes. CONCLUSION The δ ALAD genotypes did not modified the relationship between serum lead level and short term memory in gas station workers.

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Rujito, L., Setyowati, A. D., & Saien, S. (2012). Aminolevulinate dehydrogenase polymorphisms did not modified lead serum and memory relationship. Universa Medicina, 31(3), 184–191.
Review Article


Schwartz BS, Howard H. Adult lead exposure: time for change. Environ Health Perspect 2007;115:451-4.

Jacobs D, Clickner R, Zhou JY, Viet SE, Marker D, Rogers J, et al. The prevalence of lead-based paint hazards in U.S. housing. Environ Health Perspect 2002;110:599-606.

Ye X, Wong O. Lead exposure, lead poisoning, and lead regulatory standards in China, 1990-2005. Regul Toxicol Pharmacol 2006;46:157-62.

Parry J. Metal smelting plants poison hundreds of Chinese children. BMJ 2009;339:3433-8.

Pickrell J. Ten of the most polluted places on the planet. Available at : October 10, 2012.

Saepudin A. Kajian pencemaran udara akibat emisi kendaraan bermotor di DKI Jakarta. Teknol Indonesia 2005;28:29-39.

Global Agriculture Information Network. Indonesia biofuels annuals, GAIN Report ID 1134;2011.

Agency for Toxic Substances and Disease Registry. Toxicological profile for lead, 2007. Available at: Accessed September 14,2011.

Palar H. Pencemaran dan toksikologi logam berat. Jakarta: Penerbit Rineka Cipta; 2004.

Verstraeten SV, Aimo L, Oteiza PI. Aluminium and lead: molecular mechanisms of brain toxicity. Arch Toxicol 2008;82:789-802.

Kamel F, Umbach DM, Lehman TA, Park LP, Munsat TL, Shefner JM, et al. Amyotrophic lateral sclerosis, lead, and genetic susceptibility: polymorphisms in the ä-aminolevulinic acid

dehydratase and vitamin D receptor genes. Environ Health Perspect 2003;111:1335-9.

Blaylock RL. A possible central mechanism in autism spectrum disorders, Part 3: the role of excitotoxin food additives and the synergistic effects of other environmental toxins. Altern Ther Health Med 2009;15:56-60.

Carlisle JC, Dowling KC, Siegel DM, Alexeeff GV. A blood lead benchmark for assessing risks from childhood lead exposure. J Environ Sci Health A Tox Hazard Subst Environ Eng 2009;44:1200-8.

Sherwood L. Human physiology: from cells to systems 6th edition. Belmont, CA: Thomas Brooks/Cole; 2007.

Lidsky TI, Schneider JS. Lead neurotoxicity in children: basic mechanisms and clinical correlates. Brain 2003;126:5-19.

Yang Y, Wu J, Sun P. Effects of deltaaminolevulinic acid dehydratase polymorphisms on susceptibility to lead in Han subjects from Southwestern China. Int J Environ Res Public

Health 2012;9:2326-38.

Scinicariello F, Murray HE, Moffett DB, Abadin HG, Sexton MJ, Fowler BA. Lead and [delta]-aminolevulinic acid dehydratase polymorphism:where does it lead? A meta-analysis. Environ

Health Perspect 2007;115:35-41.

Winarso H. Hubungan kadar Pb darah dengan kelelahan subyektif petugas SPBU di kota Purwokerto (skripsi). Semarang: Program Studi Kesehatan Lingkungan, Politeknik Kesehatan


Hopkins MR, Ettinger AS, Hernández-Avila M, Schwartz J, Téllez-Rojo MM, Lamadrid- igueroa H, et al. Variants in iron metabolism genes predict higher blood lead levels in young

children. Environ Health Perspect 2008;116:1261-6.

Shaik AP, Jamil K. A study on the ALAD gene polymorphisms associated with lead exposure. Toxicol Ind Health 2008;24:501-6.

Shaik AP, Khan M, Jamil K. Phylogenetic analysis of ALAD and MGP genes related to lead toxicity. Toxicol Ind Health 2009;25403-9.

Torra M, Barrot C, Ortega M, Sanchez C, Xifró A, Corbella J, et al. Genetic variability of äaminolevulinic acid dehydratase (ä-ALAD) and the whole blood lead concentration in Northeast

Spain. Trace Elem Electrolytes 2006;23:5-10.

Bijoor AR, Venkatesh T. Genetic susceptibility to lead poisoning – a case report. Indian J ClinBiochem 2007;22:162-3.

Chia SE, Zhou H, Tham MT, Yap E, Viet Dong N, Hong T, et al. Possible influence of äaminolevulinic acid dehydratase polymorphism and susceptibility to renal toxicity of lead: a study

of a Vietnamese population. Environ Health Perspect 2005;113:1313–7.

Kosnett MJ. Health effects of low dose lead exposure in adults and children, and preventable risk posed by the consumption of game meat harvested with lead ammunition. In: Watson RT, Fuller M, Pokras M, Hunt WG, editors. Ingestion

of lead from spent ammunition: implications for wildlife and humans. Boise, Idaho: The Peregrine Fund;2009.p.24-33.

Atkinson RL, Atkinson RC, Hilgrad ER. Pengantar psikologi. Edisi Kedelapan Jilid 1 Jakarta: Penerbit Erlangga;2005.

Weisskopf MG, Proctor SP, Wright RO, Schwartz J, Spiro III A, Sparrow D, et al. Cumulative lead exposure and cognitive performance among elderly men. Epidemiol 2007;18:59-66.

Shih RA, Hu H, Weisskopf MG, Schwartz BS. Cumulative lead dose and cognitive function in adults: a review of studies that measured both blood lead and bone lead. Environ Health

Perspect 2007;115:483–92.

Weuve J, Korrick SA, Weisskopf MA, Ryan LM, Schwartz K, et al. Cumulative exposure to lead in relation to cognitive function in older women. Environ Health Perspect 2009;117:574-80.

Suprenant AM, Neath I, Brown GDA. Modeling age-related differences in immediate memory using SIMPLE. J Mem Lang 2006;55:572-86.