Typhonium flagelliforme decreases tyrosine kinase and Ki67 expression in mice

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Published: 2013-12-07
DOI: https://doi.org/10.18051/UnivMed.2013.v32.146-154
Keywords:
Typhonium flagelliforme, tyrosine kinase, Ki67 expression, mammary adenocarcinoma, mice

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Chodidjah Chodidjah
Department of Anatomy, Faculty of Medicine, Sultan Agung Islamic University
Edi Dharmana
Department of Parasitology, Faculty of Medicine, Diponegoro University
Hardhono Susanto
Department of Anatomy, Faculty of Medicine, Diponegoro University
Sarjadi Sarjadi
Department of Pathologic Anatomy, Diponegoro University

Abstract

Background Worldwide, breast cancer is the most frequent cancer in women after lung cancer. Treatments include surgery, radiation, immunotherapy and chemotherapy, but are not effective. Tyrosine kinase and Ki67 protein are markers of proliferation. Typhonium flagelliforme ethanol extract (TFEE) has been shown to inhibit proliferation of Michigan Cancer Foundation-7 (MCF7) cells in culture. The aim of the present study was to examine the effect of administration of TFEE on tyrosine kinase and Ki67 expression in mice. Methods This experimental study using post test randomized design with control group was conducted in 24 tumor-bearing CH3 mice. They were randomly divided into 4 groups, consisting of one control and 3 treatment groups (TI, T2, T3) treated daily for 30 days with 0.2 ml TFEE at dosages of 200, 400, and 800 mg/kgBW, respectively. On day 31 the tumor tissues were collected and their tyrosine kinase and Ki67 expression were levels assessed using ELISA and immunohistochemical staining, respectively. Tyrosine kinase and Ki67 expression levels were analyzed, respectively using Kruskal Wallis test and one-way Anova followed by Bonferroni post hoc test. Results Mean tyrosine kinase level was highest in the control group, followed by T3, T2 and T1 (p=0.019). Mean level of Ki 67 expression was highest in the control group, followed by T2, T3 and T1 (p=0.000). Conclussions Oral administration of TFEE at a dose of 200 mg/kgBW decreases tyrosine kinase levels and Ki 67 expression.

Article Details

Issue

Vol. 32 No. 3 (2013)

Section

Review Article

The journal allows the authors to hold the copyright without restrictions and allow the authors to retain publishing rights without restrictions.

How to Cite

Typhonium flagelliforme decreases tyrosine kinase and Ki67 expression in mice. (2013). Universa Medicina, 32(3), 146-154. https://doi.org/10.18051/UnivMed.2013.v32.146-154

References

Siegel R, Naishadam D, Jemal. Cancer statistic CA Cancer J Clin 2012;62:10-29.

Tjindarbumi D, Mangunkusumo R. Cancer in Indonesia: present and future. Jpn J Clin Oncol 2002;32 Suppl 1:S17-21.

Curado MP. Breast cancer in the world: incidence and mortality. Salud Publica Mex 2011;53:372–84.

Zablotska LB, Chak A, Das A, Neugut AI. Inceased risk of squamous cell esophageal cancer after adjuvant radiation. Am J Epidemiol 2005; 161:330-7.

Azab S, Al Hendy A. Signal transduction pathways in breast cancer: drug target and challenges. In: Gunduz M, Gunduz E, editors. Breast cancer: carcinogenesis, cell growth and signalling pathways. Intech;2011.p.109-38. ISBN. 970-953-307-714-7. Available at: http://www.intechopen.com/books/breast-cancer carcinogenesis-cell-growth-and-signalling-pathways/signal-transduction-pathways-in-breast-cancer-drugtargets. Accessed November 10, 2013.

Punglia RS, Kuntz K, Lee JH, Recht A. Radiation therapy plus tamoxifen versus tamoxifen alone after breast conserving surgery in postmenopausal therapy for primary breast cancer. Am J Epidemiol 2005;161:330-37.

Urruticoechea A, Smith IE, Dowsett M. Proliferation marker Ki-67 in early breast cancer. J Clin Oncol 2005:23:7212-20.

Weisner FG, Magener A, Fasching PA, Wesse F, Bani MR, Rauh C, et al. Ki-67, a prognostic nucleolar marker in routine clinical use in breast cancer patients. Breast 2009;18:135-41.

Widowati l, Mudahar H. Uji Aktivitas ekstrak etanol 50% umbi keladi tikus (Typhonium flagelliforme) terhadap sel tumor payudara (MCF7 cell line) secara in vitro. Media Litbang Kesehatan 2009;XIX:9-14.

Nobakt GM, Kadir MA, Stansias J. Analysis of preliminary phytochemical screening of Typhonium flagelliforme. Afr J Biotechnol 2010; 9:1655-7.

Lai CS, Mas RH, Nair NK, Majid MI, Mansor SM, NavarasmanV. Typhonium flagelliforme inhibits cancer cell growth in vitro and induces apoptosis: An evaluation by the bioactivity guide approach. J. Ethnopathomol 2008;118:14-20.

Mohan S, Abdul AB, Abdalwahab SI, Al-Zubairi AS, Sukari MA, Abdullah R, et al. Typhonium flagelliforme inhibits the proliferation of murine leukemia WEHI-3 cells invitro and induces apoptosis invivo. Leuk Res 2010;34:1483-92.

Dyah I, Syahbirin G, Affandi Y. Keladi tikus (Typhonium flagelliforme) sebagai anti kanker: mekanisme inhibisi ekstrak etanol dan ekstrak air terhadap aktivitas enzim tirosinkinase. Prosiding Seminar HKI, Departemen Kimia FMIPA IPB: Bogor; 2006.

Iswantini D, Syahbirin G, Pratiwi W. Daya inhibisi ekstrak air dan etanol temu putih (Curcuma zedori) terhadap aktivitas enzim tirosin kinase secara in vitro. Prosiding Seminar Nasional Sains. Penemuan Sains Dalam Kebangkitan Pertanian. FMIPA IPB: Bogor; 2008.

Genander M, Halford M, Xu N, Eriksson M, Ye Z. Dissociation of EphB signaling pathways mediating progenitor cell proliferation and tumor suppressor. Cell 2009;139:679-92.

Osaki L, Gama P. MAPKs and signal transduction in the control of gastrointestinal epithelial cell proliferation and differentiation. Int J Mol Sci 2013;14:10101-43.

Burcombe R, Wilson G, Dowsett M, Khan I, Richman PI, Dalley F, et al. Evaluation of Ki67 proliferation and apoptotic index before, during and after neoadjuvant chemotherapy for primary breast cancer. Breast Cancer Res 2006;8: R31. doi:10.1186/bcr1508.

Urruticoechea A, Smith IE, Dowsett M. Proliferation marker Ki-67 in early breast cancer. J Clin Oncol 2005;23:7212-20.

Kanadaswami C, Lee L, Lee P, Hwang J, Ke F, Huang Y. The antitumor activities of flavonoids. Invivo 2005;19:895-919.

Nishimura R, Osako T, Hayashi M, Toyosumi Y, Arima N. Ki67 as a prognostic marker according to breast cancer subtype an a predictor of recurrence time in primary breast cancer. Exp Ther Med 2010;1:747-54.

Daker M. Ahmad M, Khoo AS. Quercetin-induced inhibition and synergistic activity with cisplatin - a chemotherapeutic strategy for nasopharyngeal carcinoma cells. Cancer Cell Int Malaysia 2012;12:34.

Yoeli-Lerner M, Toker A. Akt/PkB signaling in cancer: a function in cell motility. Cell Cycle 2006;5:603-5.