pISSN 1907-3062 | eISSN 2407-2230 | Universa Medicina content is searchable on DOAJ, Google Scholar, and OAI

Mucin-1 expression in endometrium is higher in polycystic ovary syndrome than in normal women

Uki Retno Budihastuti, Sri Sulistyowati, Eriana Melinawati, Yohan Pamuji Marbun
Submission date: Monday, 16 December 2019
Published date: Wednesday, 20 May 2020
DOI: http://dx.doi.org/10.18051/UnivMed.2020.v39.74-80

Article Metrics

Abstract viewed: 116 times

Abstract


Background
The polycystic ovary syndrome (PCOS) is caused by endocrine system dysfunction in women. MUCIN-1 (MUC-1) expression is found in endometrial tissues, which leads to implantation process dysfunction because of imbalance of trophoblast adhesion process. This study was conducted to compare endometrial MUC-1 expression between PCOS and normal women considering all existing external variables.

Methods
This cross-sectional study was conducted in General Hospital Dr. Moewardi Surakarta. Endometrial samples were obtained from 30 infertile PCOS women based on Rotterdam criteria, and 30 normal women. Life style and reproductive data such as age, menstrual problems, menstrual cycle, age at menarche, and BMI were collected. Subjects underwent endometrial biopsy in luteinizing hormone (LH) secretion phase LH + 5 days to LH + 10 days for immunohistochemistry (IHC) of MUC-1 expression. An independent-t and multiple linear regression test were used to analyze the data at significance level of p<0.05.

Results
Mean MUC-1 expression in the PCOS endometrium (49.66 ± 47.79) was significantly higher than in normal women (7.66 ± 14.55) (p=0.03). Multivariate linear regression model of life style and reproductive variables with MUC-1 showed that PCOS (b=29.54; 95% CI 9.57-49.49; p=0.004) and BMI (b= 29.99; 95% CI 5.91-54.07; p=0.001) significantly increase MUC-1 expression. PCOS (Beta=0.37) was more important than BMI (Beta=0.30) in increasing the MUC-1 expression.

Conclusion
Expression of MUC-1 levels in the PCOS endometrium was higher than in normal women. This suggests that MUC-1 contributes to the unexplained reproductive failure in PCOS.

Keywords


Endometrium; MUC-1 expression; PCOS; normal women

Full Text:

PDF

References


Savaris RF, Groll JM, Young SL, et al. Progesterone resistance in PCOS endometrium: a microarray analysis in clomiphene citrate-treated and artificial menstrual cycles. J Clin Endocrinol Metab 2011;96:1737-46. doi: 10.1210/jc.2010-2600.

Li SY, Song Z, Song MJ, Qin JW, Zhao ML, Yang ZM. Impaired receptivity and decidualization in DHEA-induced PCOS mice. Sci Rep 2016;6:1–10. http://dx.doi.org/10.1038/srep38134.

Bashiri A, Halper KI, Orvieto R. Recurrent implantation failure - update overview on etiology, diagnosis, treatment and future directions. Reprod Biol Endocrinol 2018;16:1–18. doi: 10.1186/s12958-018-0414-2.

Cha J, Sun X, Dey SK. Mechanisms of implantation: strategies for successful pregnancy. Nat Med 2012;18:1754–67. http://dx.doi.org/10.1038/nm.3012.

Davidson LM, Coward K. Molecular mechanisms of membrane interaction at implantation. Birth Defects Res Part C - Embryo Today Rev 2016;108:19–32.

Piltonen TT. Polycystic ovary syndrome: endometrial markers. Best Pract Res Clin Obstet Gynaecol 2016 ;37:66-79. doi: 10.1016/j.bpobgyn.2016.03.008.

Wu F, Chen X, Liu Y, et al. Decreased MUC-1 in endometrium is an independent receptivity marker in recurrent implantation failure during implantation window. Reprod Biol Endocrinol 2018;16:1–7. doi: 10.1186/s12958-018-0379-1.

Zeng Y, Zhang Q, Zhang Y, et al. MUC-1 predicts colorectal cancer metastasis: a systematic review and meta-analysis of case controlled studies. PLoS One 2015;10:e0138049. doi:10.1371/journal.pone.0138049.

Margarit L, Taylor A, Roberts MH, et al. MUC-1 as a discriminator between endometrium from fertile and infertile patients with PCOS and endometriosis. J Clin Endocrinol Metab 2010;95:5320–9. doi: 10.1210/jc.2010-0603.

Lopes IM, Maganhin CC, Oliveira-Filho RM, et al. Histomorphometric analysis and markers of endometrial receptivity embryonic implantation in women with polycystic ovary syndrome during the treatment with progesterone. Reprod Sci 2014;21:930-8.

Baracat MCP, Serafini PC, Simões RDS, Maciel GAR, Soares JM, Baracat EC. Systematic review of cell adhesion molecules and estrogen receptor expression in the endometrium of patients with polycystic ovary syndrome. Int J Gynecol Obstet 2015;129:1–4. doi: 10.1016/j.ijgo.2014.10.022.

Hu M, Li J, Zhang Y, et al. Endometrial progesterone receptor isoforms in women with polycystic ovary syndrome. Am J Transl Res 2018;10:2696-705.

Bastu E, Mutlu MF, Yasa C, et al. Role of mucin 1 and glycodelin A in recurrent implantation failure. Fertil Steril 2015;103 : 1059-64. http://dx.doi.org/10.1016/j.fertnstert.2015.01.025 21.

Yang Y, Han Y, Wang W, et al. Assessing new terminal body and facial hair growth during pregnancy: toward developing a simplified visual scoring system for hirsutism. Fertil Steril 2016;105: 494–500. DOI: https://doi.org/10.1016/j.fertnstert.2015.10.036.

Fukui Y, Hirota Y, Matsuo M, et al. Uterine receptivity, embryo attachment, and embryo invasion: Multistep processes in embryo implantation. Reprod Med Biol 2019;18:234–40. doi: 10.1002/rmb2.12280.

Parks JC, McCallie BR, Patton AL, et al. The impact of infertility diagnosis on embryo-endometrial dialogue. Reproduction 2018;543–52. doi: 10.1530/REP-17-0566.

Raheem KA. Cytokines, growth factors and macromolecules as mediators of implantation in mammalian species. Int J Vet Sci Med 2018;6 Sup1:S6–14. https://doi.org/10.1016/j.ijvsm.2017.12.001.

Craciunas L, Gallos I, Chu J, et al. Conventional and modern markers of endometrial receptivity: a systematic review and meta-analysis. Hum Reprod Update 2019;25:202–23. doi: 10.1093/humupd/dmy044.

Altmäe S, Koel M, Võsa U, et al. Meta-signature of human endometrial receptivity: a meta-analysis and validation study of transcriptomic biomarkers. Sci Rep 2017;7: 10077. doi: 10.1038/s41598-017-10098-3.

Redzovic A, Laskarin G, Dominovic M, Haller M, Rukavina D. Mucins help to avoid alloreactivity at the maternal fetal interface. Clinl Develop Immunol 2013, Article ID 542152, 9 pages. http://dx.doi.org/10.1155/2013/54215.

Shen F, Yan C, Liu M, Feng Y, Chen Y. Decreased expression of mucin-1 in endometriosis endometrium correlated with progesterone receptor B involved in infertility. Arch Gynecol Obstet 2015;291:439–45. DOI: 10.1007/s00404-014-3419-x.


Refbacks

  • There are currently no refbacks.


Copyright (c) 2020 Uki Retno Budihastuti, Sri Sulistyowati, Eriana Melinawati, Yohan Pamuji Marbun

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Creative Commons License
Universa Medicina by Faculty of Medicine, Trisakti University is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Based on a work at https://univmed.org/ejurnal/index.php/medicina/


                Scilit