Alkaline peptone water plus 0.5% agar suitable for transport of Vibrio cholerae

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Published: Aug 23, 2011
DOI: https://doi.org/10.18051/UnivMed.2011.v30.95-101 Universa Medicina
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Alkaline peptone water agar Vibrio cholerae rectal swab

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Meiyanti Meiyanti
Department of Pharmacology, Medical Faculty, Trisakti University
Oktavianus Ch. Salim
Department of Public Health, Medical Faculty, Trisakti University Jakarta
Julius E. Surjawidjaja
Department of Microbiology, Medical Faculty, Trisakti University Jakarta
Murad Lesmana
Department of Microbiology, Medical Faculty, Trisakti University Jakarta

Abstract

Microbiological procedures for V. cholerae isolation from clinical specimens are important factors in clinical and epidemiological management of cholera. The standard preservation medium for enteric pathogenic bacteria, including V. cholerae, is Cary-Blair medium (CB), a semisolid medium for preservation and transport of specimens containing intestinal bacteria. A special medium for Vibrio organisms is alkaline peptone water (APW), which is both a transport and an enrichment medium. The purpose of this study was to ascertain the suitability of APW supplemented with 0.5% agar (APW-0.5) as a sensititive preservation-transport medium for rectal swab specimens for isolation of V. cholerae. A total of 144 paired rectal swab specimens were collected from children and adults with acute diarrhea. Of each specimen pair, one was placed in CB and the other in APW-0.5, from which they were plated out to thiosulfate citrate bile sucrose (TCBS) agar. Altogether, from both CB and APW-0.5 transported specimens, V. cholerae non-O1 was present in 29 (20.1%) specimens, while only 2 (1.4%) specimens were positive in CB and 9 (6.3%) positive in APW-0.5 transported specimens. The number of V. cholerae non-O1 isolates from APW-0.5 transported specimens was significantly higher (p=0.000) as compared to that from Cary-Blair transported specimens. It may be concluded that for isolation of V. cholerae, specimen transport in APW-0.5 medium was more effective than transport in Cary-Blair medium.

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How to Cite
Meiyanti, M., Salim, O. C., Surjawidjaja, J. E., & Lesmana, M. (2011). Alkaline peptone water plus 0.5% agar suitable for transport of Vibrio cholerae. Universa Medicina, 30(2), 95–101. https://doi.org/10.18051/UnivMed.2011.v30.95-101
Issue
Vol. 30 No. 2 (2011)
Section
Review Article

The journal allows the authors to hold the copyright without restrictions and allow the authors to retain publishing rights without restrictions.

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